The dichloroacetamide safener benoxacor is enantioselectively metabolized by monkey liver microsomes and cytosol.
Biotransformation
Carboxylesterase
Chiral
Cytochrome P450 enzyme
Enantiomer
Glutathione-S-transferase
Journal
Environmental toxicology and pharmacology
ISSN: 1872-7077
Titre abrégé: Environ Toxicol Pharmacol
Pays: Netherlands
ID NLM: 9612020
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
received:
31
08
2022
revised:
26
10
2022
accepted:
27
10
2022
pubmed:
8
11
2022
medline:
29
11
2022
entrez:
7
11
2022
Statut:
ppublish
Résumé
The metabolism and toxicity of current-use herbicide safeners remain understudied. We investigated the enantioselective metabolism of the safener benoxacor in Rhesus monkey subcellular fractions. Benoxacor was incubated with liver microsomes and cytosol from female and male monkeys (≤30 min). Benoxacor levels and enantiomeric fractions were determined with gas chromatography. Benoxacor was metabolized by microsomal cytochrome P450 enzymes (CYPs), cytosolic glutathione-S-transferases (GSTs), and microsomal and cytosolic carboxylesterase (CESs). CES-mediated microsomal metabolism followed the order males > females, whereas the CYP-mediated clearance followed the order females > males. CYP-mediated metabolism initially resulted in an enrichment of the second eluting benoxacor enantiomer (E
Identifiants
pubmed: 36341964
pii: S1382-6689(22)00201-0
doi: 10.1016/j.etap.2022.104008
pii:
doi:
Substances chimiques
2,2-dichloroacetamide
I202LTA03D
benoxacor
UAI2652GEV
Acetamides
0
Cytochrome P-450 Enzyme System
9035-51-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104008Informations de copyright
Copyright © 2022 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Hans-Joachim Lehmler reports financial support was provided by National Institute of Environmental Health Sciences. David Cwiertny reports financial support was provided by National Science Foundation. Hans-Joachim Lehmler reports financial support was provided by National Science Foundation.