Postmortem 7T MRI for guided histopathology and evaluation of cerebrovascular disease.
7T MRI
Cerebrovascular disease
Enlarged perivascular spaces
Histology
Neuroimaging
Postmortem
White matter hyperintensity
Journal
Journal of neuropathology and experimental neurology
ISSN: 1554-6578
Titre abrégé: J Neuropathol Exp Neurol
Pays: England
ID NLM: 2985192R
Informations de publication
Date de publication:
19 12 2022
19 12 2022
Historique:
pubmed:
8
11
2022
medline:
22
12
2022
entrez:
7
11
2022
Statut:
ppublish
Résumé
Postmortem (PM) magnetic resonance imaging (MRI) can serve as a bridge between in vivo imaging and histology by connecting MRI observed macrostructural findings to histological staining and microstructural changes. Data were acquired from 20 formalin-fixed brains including T2, T1, PD, and T2*-weighted images of left hemispheres and 6-mm-thick coronal slices. Tissue slices were bisected, aligned to MR images and used to guide histological sampling. Markers of myelin and oligodendroglia alterations were semiquantitatively rated and compared within white matter hyperintensities (WMHs) and normal-appearing white matter. Tissue priors were created from 3T in vivo data and used to guide segmentation of WMH. PM WMH and hemisphere volumes were compared to volumes derived from in vivo data. PM T2 WMH and T1 hemisphere volumes were correlated with in vivo 3T FLAIR WMH and T1 hemisphere volumes. WMH showed significant myelin loss, decreased GFAP expression and increased vimentin expression. MR-visible perivascular spaces and cortical microvascular lesions were successfully captured on histopathological sections. PM MRI can quantify cerebrovascular disease burden and guide tissue sampling, allowing for more comprehensive characterization of cerebrovascular disease that may be used to study etiologies of age-related cognitive change.
Identifiants
pubmed: 36343095
pii: 6809038
doi: 10.1093/jnen/nlac103
pmc: PMC9764082
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
57-70Subventions
Organisme : NIA NIH HHS
ID : P30 AG066518
Pays : United States
Organisme : VA
Pays : United States
Organisme : NIH HHS
ID : R01 AG056712
Pays : United States
Organisme : NIA NIH HHS
ID : P30 AG008017
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG036772
Pays : United States
Organisme : NIA NIH HHS
ID : K01 AG059842
Pays : United States
Organisme : NINDS NIH HHS
ID : P30 NS061800
Pays : United States
Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of American Association of Neuropathologists, Inc.
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