CD1d-dependent rewiring of lipid metabolism in macrophages regulates innate immune responses.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
07 11 2022
Historique:
received: 08 03 2022
accepted: 27 10 2022
entrez: 7 11 2022
pubmed: 8 11 2022
medline: 10 11 2022
Statut: epublish

Résumé

Alterations in cellular metabolism underpin macrophage activation, yet little is known regarding how key immunological molecules regulate metabolic programs in macrophages. Here we uncover a function for the antigen presenting molecule CD1d in the control of lipid metabolism. We show that CD1d-deficient macrophages exhibit a metabolic reprogramming, with a downregulation of lipid metabolic pathways and an increase in exogenous lipid import. This metabolic rewiring primes macrophages for enhanced responses to innate signals, as CD1d-KO cells show higher signalling and cytokine secretion upon Toll-like receptor stimulation. Mechanistically, CD1d modulates lipid import by controlling the internalization of the lipid transporter CD36, while blocking lipid uptake through CD36 restores metabolic and immune responses in macrophages. Thus, our data reveal CD1d as a key regulator of an inflammatory-metabolic circuit in macrophages, independent of its function in the control of T cell responses.

Identifiants

pubmed: 36344546
doi: 10.1038/s41467-022-34532-x
pii: 10.1038/s41467-022-34532-x
pmc: PMC9640663
doi:

Substances chimiques

Antigens, CD1d 0
Lipids 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6723

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/T013710/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S005560/1
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C604/A25135
Pays : United Kingdom

Informations de copyright

© 2022. The Author(s).

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Auteurs

Phillip M Brailey (PM)

The Peter Gorer Department of Immunobiology, King's College London, London, UK.
The Francis Crick Institute, London, UK.

Lauren Evans (L)

The Peter Gorer Department of Immunobiology, King's College London, London, UK.
The Francis Crick Institute, London, UK.

Juan Carlos López-Rodríguez (JC)

The Peter Gorer Department of Immunobiology, King's College London, London, UK.
The Francis Crick Institute, London, UK.

Anthony Sinadinos (A)

The Peter Gorer Department of Immunobiology, King's College London, London, UK.
The Francis Crick Institute, London, UK.

Victoria Tyrrel (V)

School of Medicine, Cardiff University, Cardiff, UK.

Gavin Kelly (G)

The Francis Crick Institute, London, UK.

Valerie O'Donnell (V)

School of Medicine, Cardiff University, Cardiff, UK.

Peter Ghazal (P)

School of Medicine, Cardiff University, Cardiff, UK.

Susan John (S)

The Peter Gorer Department of Immunobiology, King's College London, London, UK.

Patricia Barral (P)

The Peter Gorer Department of Immunobiology, King's College London, London, UK. patricia.barral@kcl.ac.uk.
The Francis Crick Institute, London, UK. patricia.barral@kcl.ac.uk.

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