Multi-glycomic analysis of spheroid glycocalyx differentiates 2- and 3-dimensional cell models.
3D cell culture model
colon cancer
glycomics
mass spectrometry
Journal
Glycobiology
ISSN: 1460-2423
Titre abrégé: Glycobiology
Pays: England
ID NLM: 9104124
Informations de publication
Date de publication:
08 01 2023
08 01 2023
Historique:
received:
04
08
2022
revised:
10
09
2022
accepted:
26
09
2022
pubmed:
9
11
2022
medline:
12
1
2023
entrez:
8
11
2022
Statut:
ppublish
Résumé
A multi-glycomic method for characterizing the glycocalyx was employed to identify the difference between 2-dimensional (2D) and 3-dimensional (3D) culture models with two human colorectal cancer cell lines, HCT116 and HT29. 3D cell cultures are considered more representative of cancer due to their ability to mimic the microenvironment found in tumors. For this reason, they have become an important tool in cancer research. Cell-cell interactions increase in 3D models compared to 2D, indeed significant glycomic changes were observed for each cell line. Analyses included the N-glycome, O-glycome, glycolipidome, glycoproteome, and proteome providing the most extensive characterization of the glycocalyx between 3D and 2D thus far. The different glycoconjugates were affected in different ways. In the N-glycome, the 3D cells increased in high-mannose glycosylation and in core fucosylation. Glycolipids increased in sialylation. Specific glycoproteins were found to increase in the 3D cell, elucidating the pathways that are affected between the two models. The results show large structural and biological changes between the 2 models suggesting that the 2 are indeed very different potentially affecting individual outcomes in the study of diseases.
Identifiants
pubmed: 36345209
pii: 6809075
doi: 10.1093/glycob/cwac075
pmc: PMC9829041
doi:
Substances chimiques
Glycoproteins
0
Polysaccharides
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2-16Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM049077
Pays : United States
Informations de copyright
© The Author(s) 2022. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
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