Application of a Cholesterol-Based Stationary Phase for the Analysis of Brevetoxins.
potential drug
silica hydride stationary phase
solvent-based selectivity
temperature-based selectivity
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
revised:
20
10
2022
received:
16
08
2022
accepted:
07
11
2022
pubmed:
13
11
2022
medline:
1
2
2023
entrez:
12
11
2022
Statut:
ppublish
Résumé
A high-performance liquid chromatography protocol for the analysis of brevetoxins has been developed using a silica hydride-based cholesterol column. Brevetoxins are neurotoxins produced by harmful algae that have additional potential as drugs for a number of illnesses/diseases. To develop the optimum conditions, a number of different experimental approaches were tested. These include isocratic and gradient elution, different organic mobile phase components, and temperature variations. A separate protocol was developed for the compounds brevenal and brevenol, also produced by the same algae that make brevetoxins. Brevenal is a natural product under investigation as a therapy for chronic respiratory diseases, such as cystic fibrosis or asthma. The goal of this study was to provide a protocol for the analysis of these compounds that could be further developed into a validated method depending on a particular laboratory's capabilities and to highlight some of the unique features of the cholesterol stationary phase.
Identifiants
pubmed: 36369995
doi: 10.1002/jssc.202200666
doi:
Substances chimiques
brevetoxin
98225-48-0
Marine Toxins
0
Oxocins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2200666Informations de copyright
© 2022 Wiley-VCH GmbH.
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