LACTB exerts tumor suppressor properties in epithelial ovarian cancer through regulation of Slug.


Journal

Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869

Informations de publication

Date de publication:
01 2023
Historique:
received: 03 05 2022
revised: 24 10 2022
accepted: 25 10 2022
entrez: 14 11 2022
pubmed: 15 11 2022
medline: 18 11 2022
Statut: epublish

Résumé

Epithelial-mesenchymal transition (EMT) is a cellular mechanism used by cancer cells to acquire migratory and stemness properties. In this study, we show, through in vitro, in vivo, and 3D culture experiments, that the mitochondrial protein LACTB manifests tumor suppressor properties in ovarian cancer. We show that LACTB is significantly down-regulated in epithelial ovarian cancer cells and clinical tissues. Re-expression of LACTB negatively effects the growth of cancer cells but not of non-tumorigenic cells. Mechanistically, we show that LACTB leads to differentiation of ovarian cancer cells and loss of their stemness properties, which is achieved through the inhibition of the EMT program and the LACTB-dependent down-regulation of Snail2/Slug transcription factor. This study uncovers a novel role of LACTB in ovarian cancer and proposes new ways of counteracting the oncogenic EMT program in this model system.

Identifiants

pubmed: 36375842
pii: 6/1/e202201510
doi: 10.26508/lsa.202201510
pmc: PMC9664245
pii:
doi:

Substances chimiques

beta-Lactamases EC 3.5.2.6
LACTB protein, human EC 3.4.-
Membrane Proteins 0
Mitochondrial Proteins 0
Snail Family Transcription Factors 0
SNAI2 protein, human 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Commentaires et corrections

Type : ErratumIn

Informations de copyright

© 2022 Cutano et al.

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Auteurs

Valentina Cutano (V)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.

Jessica Marianne Ferreira Mendes (JM)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.

Sara Escudeiro-Lopes (S)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.
Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic.

Susana Machado (S)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.

Judith Vinaixa Forner (J)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.

Juan M Gonzales-Morena (JM)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.

Martin Prevorovsky (M)

Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic.

Viacheslav Zemlianski (V)

Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic.

Yuxiong Feng (Y)

Zhejiang Provincial Key Laboratory of Pancreatic Disease, First Affiliated Hospital, and Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, China.

Petra Kralova Viziova (P)

The Czech Center for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Vestec, Czech Republic.

Andrea Hartmanova (A)

The Czech Center for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Vestec, Czech Republic.

Beata Malcekova (B)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.

Pavel Jakoube (P)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.
Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic.

Sonia Iyer (S)

Whitehead Institute for Biomedical Research, Cambridge, MA, USA.

Zuzana Keckesova (Z)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic keckesova@uochb.cas.cz.

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Classifications MeSH