Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation.

bone morphogenic protein 2 chondroitin sulfate collagen I human adipose-derived mesenchymal stem cells lipoplexes osteogenic differentiation polyelectrolyte multilayers

Journal

Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613

Informations de publication

Date de publication:
02 2023
Historique:
revised: 25 10 2022
received: 05 08 2022
pubmed: 16 11 2022
medline: 22 2 2023
entrez: 15 11 2022
Statut: ppublish

Résumé

A gene-activated surface coating is presented as a strategy to design smart biomaterials for bone tissue engineering. The thin-film coating is based on polyelectrolyte multilayers composed of collagen I and chondroitin sulfate, two main biopolymers of the bone extracellular matrix, which are fabricated by layer-by-layer assembly. For further functionalization, DNA/lipid-nanoparticles (lipoplexes) are incorporated into the multilayers. The polyelectrolyte multilayer fabrication and lipoplex deposition are analyzed by surface sensitive analytical methods that demonstrate successful thin-film formation, fibrillar structuring of collagen, and homogenous embedding of lipoplexes. Culture of mesenchymal stem cells on the lipoplex functionalized multilayer results in excellent attachment and growth of them, and also, their ability to take up cargo like fluorescence-labelled DNA from lipoplexes. The functionalization of the multilayer with lipoplexes encapsulating DNA encoding for transient expression of bone morphogenetic protein 2 induces osteogenic differentiation of mesenchymal stem cells, which is shown by mRNA quantification for osteogenic genes and histochemical staining. In summary, the novel gene-functionalized and extracellular matrix mimicking multilayer composed of collagen I, chondroitin sulfate, and lipoplexes, represents a smart surface functionalization that holds great promise for tissue engineering constructs and implant coatings to promote regeneration of bone and other tissues.

Identifiants

pubmed: 36377486
doi: 10.1002/adhm.202201978
doi:

Substances chimiques

Chondroitin Sulfates 9007-28-7
Polyelectrolytes 0
Collagen 9007-34-5
Collagen Type I 0
DNA 9007-49-2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2201978

Informations de copyright

© 2022 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.

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Auteurs

Catharina Husteden (C)

Institute of Pharmacy, Department of Medicinal Chemistry, Martin Luther University Halle-Wittenberg, Wolfgang-Langenbeck-Str. 4, 06120, Halle (Saale), Germany.

Yazmin A Brito Barrera (YA)

Institute of Pharmacy, Department of Biomedical Materials, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Str. 4, 06120, Halle (Saale), Germany.

Sophia Tegtmeyer (S)

Institute of Pharmacy, Department of Medicinal Chemistry, Martin Luther University Halle-Wittenberg, Wolfgang-Langenbeck-Str. 4, 06120, Halle (Saale), Germany.

João Borges (J)

Department of Chemistry, CICECO - Aveiro Institute of Materials, University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal.

Julia Giselbrecht (J)

Institute of Pharmacy, Department of Medicinal Chemistry, Martin Luther University Halle-Wittenberg, Wolfgang-Langenbeck-Str. 4, 06120, Halle (Saale), Germany.

Matthias Menzel (M)

Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems (IMWS), Walter-Hülse-Str. 1, 06120, Halle (Saale), Germany.

Andreas Langner (A)

Institute of Pharmacy, Department of Medicinal Chemistry, Martin Luther University Halle-Wittenberg, Wolfgang-Langenbeck-Str. 4, 06120, Halle (Saale), Germany.

João F Mano (JF)

Department of Chemistry, CICECO - Aveiro Institute of Materials, University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal.

Christian E H Schmelzer (CEH)

Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems (IMWS), Walter-Hülse-Str. 1, 06120, Halle (Saale), Germany.

Christian Wölk (C)

Institute of Pharmacy, Pharmaceutical Technology, Faculty of Medicine, Leipzig University, 04317, Leipzig, Germany.

Thomas Groth (T)

Institute of Pharmacy, Department of Biomedical Materials, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Str. 4, 06120, Halle (Saale), Germany.
Interdisciplinary Center of Materials Science, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Str. 4, 06120, Halle (Saale), Germany.

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