Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases.
OTS1
Protease assay
Protein expression
Recombinant protein
SUMO
SUMO protease
SUMOylation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
22
11
2022
pubmed:
23
11
2022
medline:
25
11
2022
Statut:
ppublish
Résumé
The conjugation of SUMO can profoundly change the behavior of substrate proteins, impacting a wide variety of cellular responses. SUMO proteases are emerging as key regulators of plant adaptation to its environment because of their instrumental role in the SUMO deconjugation process. Here we describe how to express, purify, and determine SUMO deconjugation activity of a plant SUMO protease.
Identifiants
pubmed: 36413314
doi: 10.1007/978-1-0716-2784-6_9
doi:
Substances chimiques
Peptide Hydrolases
EC 3.4.-
Endopeptidases
EC 3.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
109-119Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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