RNA-activated protein cleavage with a CRISPR-associated endopeptidase.
Humans
CRISPR-Cas Systems
Cryoelectron Microscopy
Endopeptidases
/ chemistry
RNA, Bacterial
/ chemistry
CRISPR-Associated Proteins
/ chemistry
Proteolysis
Deltaproteobacteria
/ enzymology
RNA, Guide, Kinetoplastida
/ chemistry
Sigma Factor
/ metabolism
Transcription, Genetic
Substrate Specificity
Allosteric Regulation
Bacterial Proteins
/ chemistry
Enzyme Activation
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
25 11 2022
25 11 2022
Historique:
entrez:
24
11
2022
pubmed:
25
11
2022
medline:
29
11
2022
Statut:
ppublish
Résumé
In prokaryotes, CRISPR-Cas systems provide adaptive immune responses against foreign genetic elements through RNA-guided nuclease activity. Recently, additional genes with non-nuclease functions have been found in genetic association with CRISPR systems, suggesting that there may be other RNA-guided non-nucleolytic enzymes. One such gene from
Identifiants
pubmed: 36423276
doi: 10.1126/science.add7450
pmc: PMC10028731
mid: NIHMS1863051
doi:
Substances chimiques
Endopeptidases
EC 3.4.-
RNA, Bacterial
0
CRISPR-Associated Proteins
0
RNA, Guide
0
Sigma Factor
0
Bacterial Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
874-881Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NHLBI NIH HHS
ID : DP1 HL141201
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG009761
Pays : United States
Commentaires et corrections
Type : CommentIn
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