Estimation of some trace metals, bioactive compounds, curative antimicrobial and antioxidant agents from Russula foetens and Russula cf. foetentoides.

The current study is an attempt to estimate minerals, antioxidant and antimicrobial activities of two medicinal mushrooms (Russula foetens and Russula cf. foetentoides) along with their mycochemicals. Among the metal analysis, iron was most abundantly (7.428mg/g) present in R. cf. foetentoides. The mycochemical screening revealed the presence of active secondary metabolites and phenolic composition by HPLC showed five stable compounds with maximum concentration of gallic acid (22.31%). The highest antibacterial activity (27.8±0.152mm) was displayed in ethanol macerate of R. foetens in contradiction of Staphylococcus aureus. Similarly, highest antifungal potential was exhibited by R. foetens (ethanolic macerate) against Fusarium equiseti with zone of inhibition 23±0.572mm. The antioxidant potential was assessed by five assays and the R. foetens exhibited maximum % inhibition (43.045±0.037%) by DPPH radical protocol. Maximum % inhibition by ABTS protocol was exhibited by R. cf. foetentoides i.e., 88.057±0.021. The highest reducing potential was revealed in R. foetens (0.684±0.001mmol/L of FeSO4). The highest flavonoids and total phenolic contents (TPC) were shown by R. cf. foetentoides i.e., 0.115±0.002mg/100g of Catechin and 0.064±0.001mg/100g of GAE (gallic acid equivalents), respectively. All the obtained results were analyzed statistically by applying the ANOVA to determine the significant and non-significant ranges.