Description and Phylogenetic Relationships of Anhingatrema n. gen. (Digenea: Diplostomidae) with Two New Species from New World Anhingas (Aves: Anhingidae).
Anhinga anhinga
Anhingatrema cararai n. sp.
Anhingatrema n. gen.
Anhingatrema overstreeti n. sp.
Diplostomidae
Molecular phylogeny
Journal
Acta parasitologica
ISSN: 1896-1851
Titre abrégé: Acta Parasitol
Pays: Switzerland
ID NLM: 9301947
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
received:
06
09
2022
accepted:
08
11
2022
pubmed:
2
12
2022
medline:
16
3
2023
entrez:
1
12
2022
Statut:
ppublish
Résumé
The Diplostomidae is a globally distributed family of digeneans that parasitize a wide variety of tetrapod definitive hosts. Recent molecular phylogenetic studies have revealed unknown diplostomid diversity in avian hosts throughout the New World. Herein, we provide descriptions of a novel genus of diplostomids with two new species. Two species of diplostomids belonging to the new genus were collected from anhinga birds in Mississippi (USA) and Brazil. Partial nuclear 28S ribosomal and mitochondrial cox1 genes were sequenced. Ribosomal data were used for phylogenetic inference. Both species of Anhingatrema n. gen. were positioned in a 100% supported, monophyletic clade in the phylogenetic tree. The molecular phylogenetic position and a combination of morphological features (e.g., presence of pseudosuckers, testes shape and orientation) supported erection of the new genus. Anhingatrema overstreeti n. sp. and Anhingatrema cararai n. sp. are morphologically similar, but differ in size of and ratios associated with pseudosuckers. The two species differ by 2% of 28S sequences and 13.8% of cox1 sequences. Comparison of DNA sequences revealed that Diplostomidae gen. sp. in GenBank (MZ314151) is conspecific with An. overstreeti n. sp. Anhingatrema n. gen. is the sixth genus of diplostomids known from anhingas worldwide. Anhingatrema cararai n. sp. is the first diplostomid to be reported from anhingas in South America. Combined with previous studies, the molecular phylogenies revealed at least two host switches to anhingas from other birds during the evolutionary history of the Diplostomidae.
Identifiants
pubmed: 36456776
doi: 10.1007/s11686-022-00643-0
pii: 10.1007/s11686-022-00643-0
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
159-171Subventions
Organisme : National Science Foundation
ID : DEB-1120734
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : 88887.342366/2019-00
Informations de copyright
© 2022. The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences.
Références
Niewiadomska K (2002) Family Diplostomidae Poirier, 1886. In: Gibson DI, Jones A, Bray RA (eds) Keys to the Trematoda, vol 1. CAB International and The Natural History Museum, London, pp 167–196
doi: 10.1079/9780851995472.0167
Dubois G, Pearson JC (1967) Quelques Strigeida (Trematoda) D’Australie II. Bull Soc Neuchâtel Sci Nat 90:185–204. https://doi.org/10.5169/seals-88970
doi: 10.5169/seals-88970
Dubois G (1968) Synopsis des Strigeidae et des Diplostomatidae (Trematoda). Mem Soc Sci Nat Neuchâtel 10:1–258
Mehra RK (1962) Two new species of the genus Tylodelphys Diesing, 1850 (Trematoda: Diplostomidae Poirier, 1886) from the Indian snake bird Anhinga melanogaster Pennant. Proc Natl Acad Sci India 32:50–56
Forrester DJ, Spalding MG (2003) Parasites and diseases of wild birds in Florida. University Press of Florida, Gainesville
Ukoli F (1968) Three new trematode parasites of the African darter, Anhinga rufa rufa (Lacepéde and Daudin, 1802) in Ghana. J Helminthol 42:179–192. https://doi.org/10.1017/S0022149X00027322
doi: 10.1017/S0022149X00027322
Heneberg P, Sitko J, Těšínský M (2020) Paraphyly of Conodiplostomum Dubois, 1937. Parasitol Int 76:102033. https://doi.org/10.1016/j.parint.2019.102033
doi: 10.1016/j.parint.2019.102033
pubmed: 31809881
Achatz TJ, Chermak TP, Martens JR, Pulis EE, Fecchio A, Bell JA, Greiman SE, Cromwell KJ, Brant SV, Kent ML, Tkach VV (2021) Unravelling the diversity of the Crassiphialinae (Digenea: Diplostomidae) with molecular phylogeny and descriptions of five new species. Curr Res Parasitol Vector-Borne Dis 1:100051. https://doi.org/10.1016/j.crpvbd.2021.100051
doi: 10.1016/j.crpvbd.2021.100051
pubmed: 35284861
pmcid: 8906103
Fernandes BMM, Justo MCN, Cárdenas MQ, Cohen SC (2015) South American trematodes parasites of birds and mammals. Oficina de Livros, Rio de Janeiro
Overstreet RM, Curran SS, Pote LM, King DT, Blend CK, Grater WD (2002) Bolbophorus damnificus n. sp. (Digenea: Bolbophoridae) from the channel catfish Ictalurus punctatus and American white pelican Pelecanus erythrorhynchos in the USA based on life-cycle and molecular data. Syst Parasitol 52:81–96. https://doi.org/10.1023/A:1015696622961
doi: 10.1023/A:1015696622961
pubmed: 12075128
Locke SA, Van Dam AR, Caffara M, Pinto HA, López-Hernández D, Blanar CA (2018) Validity of the Diplostomoidea and Diplostomida (Digenea, Platyhelminthes) upheld in phylogenomic analysis. Int J Parasitol 48:1043–1059. https://doi.org/10.1016/j.ijpara.2018.07.001
doi: 10.1016/j.ijpara.2018.07.001
pubmed: 30347194
Locke SA, Drago FB, Núñez V, Rangel e Souza GT, Takemoto RM (2020) Phylogenetic position of Diplostomum spp. from New World herons based on complete mitogenomes, rDNA operons, and DNA barcodes, including a new species with partially elucidated life cycle. Parasitol Res 119:2129–2137. https://doi.org/10.1007/s00436-020-06713-4
doi: 10.1007/s00436-020-06713-4
pubmed: 32472382
López-Jiménez A, Pérez-Ponce de León G, García-Varela M (2018) Molecular data reveal high diversity of Uvulifer (Trematoda: Diplostomidae) in Middle America, with the description of a new species. J Helminthol 92:725–739. https://doi.org/10.1017/S0022149X17000888
doi: 10.1017/S0022149X17000888
pubmed: 29017625
Sereno-Uribe AL, Anrade-Gómez L, Ponce de Leon GP, García-Varela M (2019) Exploring the genetic diversity of Tylodelphys (Diesing, 1850) metacercariae in the cranial and body cavities of Mexican freshwater fishes using nuclear and mitochondrial DNA sequences, with the description of a new species. Parasitol Res 118:203–217. https://doi.org/10.1007/s00436-018-6168-0
doi: 10.1007/s00436-018-6168-0
pubmed: 30552574
Achatz TJ, Martens JR, Kostadinova A, Pulis EE, Orlofske SA, Bell JA, Fecchio A, Oyarzún-Ruiz P, Syrota YY, Tkach VV (2022) Molecular phylogeny of Diplostomum, Tylodelphys, Austrodiplostomum and Paralaria (Digenea: Diplostomidae) necessitates systematic changes and reveals a history of evolutionary host switching events. Int J Parasitol 52:47–63. https://doi.org/10.1016/j.ijpara.2021.06.002
doi: 10.1016/j.ijpara.2021.06.002
pubmed: 34371018
Lutz HL, Tkach VV, Weckstein JD (2017) Methods for specimen-based studies of avian symbionts. In: Webster M (ed) The role of collections in ornithology: the extended specimen. Studies in avian biology. CRC Press, Florida, pp 127–183
Tkach VV, Pawlowski J (1999) A new method of DNA extraction from the ethanol-fixed parasitic worms. Acta Parasitol 44:147–148
Tkach VV, Littlewood DTJ, Olson PD, Kinsella JM, Swiderski Z (2003) Molecular phylogenetic analysis of the Microphalloidea Ward, 1901 (Trematoda: Digenea). Syst Parasitol 56:1–15. https://doi.org/10.1023/A:1025546001611
doi: 10.1023/A:1025546001611
pubmed: 12975618
Achatz TJ, Brito ES, Fecchio A, Tkach VV (2021) Description and phylogenetic position of a new species of Herpetodiplostomum from Phrynops geoffroanus in Brazil and a re-evaluation Cheloniodiplostomum. J Parasitol 107:455–462. https://doi.org/10.1645/21-18
doi: 10.1645/21-18
pubmed: 34086924
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev/msw054
doi: 10.1093/molbev/msw054
pubmed: 27004904
pmcid: 8210823
Achatz TJ, Pulis EE, Junker K, Tran BT, Snyder SD, Tkach VV (2019) Molecular phylogeny of the Cyathocotylidae (Digenea, Diplostomoidea) necessitates systematic changes and reveals a history of host and environment switches. Zool Scr 48:545–556. https://doi.org/10.1111/zsc.12360
doi: 10.1111/zsc.12360
pubmed: 31937984
pmcid: 6959977
Ronquist F, Huelsenbeck JP (2003) MRBAYES 3: bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. https://doi.org/10.1093/bioinformatics/btg180
doi: 10.1093/bioinformatics/btg180
pubmed: 12912839
Hernández-Mena DI, García-Varela M, Pérez-Ponce de León G (2017) Filling the gaps in the classification of the Digenea Carus, 1863: systematic position of the Proterodiplostomidae Dubois, 1936 within the superfamily Diplostomoidea Poirier, 1886, inferred from nuclear and mitochondrial DNA sequences. Syst Parasitol 94:833–848. https://doi.org/10.1007/s11230-017-9745-1
doi: 10.1007/s11230-017-9745-1
pubmed: 28822036
Achatz TJ, Martens JM, Kudlai O, Junker K, Boe NW, Tkach VV (2022) A new genus of diplostomids (Digenea: Diplostomoidea) from Nile crocodile in South Africa with an updated key to diplostomid genera. J Parasitol 108:453–466. https://doi.org/10.1645/22-23
doi: 10.1645/22-23
pubmed: 36223170
Hernández-Mena DI, García-Prieto L, García-Varela M (2014) Morphological and molecular differentiation of Parastrigea (Trematoda: Strigeidae) from Mexico, with the description of a new species. Parasitol Int 63:315–323. https://doi.org/10.1016/j.parint.2013.11.012
doi: 10.1016/j.parint.2013.11.012
pubmed: 24309555
Gordy MA, Locke SA, Rawlings TA, Lapierre AR, Hanington PC (2017) Molecular and morphological evidence for nine species in North American Australapatemon (Sudarikov, 1959): a phylogeny expansion with description of the zygocercous Australapatemon mclaughlini n. sp. Parasitol Res 116:2181–2198. https://doi.org/10.1007/s00436-017-5523-x
doi: 10.1007/s00436-017-5523-x
pubmed: 28623502