The Yin-Yang of the Green Fluorescent Protein: Impact on Saccharomyces cerevisiae stress resistance.
Green fluorescent protein
Heating kinetics
Membrane patches
Oxidation
Saccharomyces cerevisiae
Stress granules
Journal
Journal of photochemistry and photobiology. B, Biology
ISSN: 1873-2682
Titre abrégé: J Photochem Photobiol B
Pays: Switzerland
ID NLM: 8804966
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
received:
25
07
2022
revised:
09
11
2022
accepted:
21
11
2022
pubmed:
3
12
2022
medline:
28
12
2022
entrez:
2
12
2022
Statut:
ppublish
Résumé
Although fluorescent proteins are widely used as biomarkers (Yin), no study focuses on their influence on the microbial stress response. Here, the Green Fluorescent Protein (GFP) was fused to two proteins of interest in Saccharomyces cerevisiae. Pab1p and Sur7p, respectively involved in stress granules structure and in Can1 membrane domains. These were chosen since questions remain regarding the understanding of the behavior of S. cerevisiae facing different heat kinetics or oxidative stresses. The main results showed that Pab1p-GFP fluorescent mutant displayed a higher resistance than that of the wild type under a heat shock. Moreover, fluorescent mutants exposed to oxidative stresses displayed changes in the cultivability compared to the wild type strain. In silico approaches showed that the presence of the GFP did not influence the structure and so the functionality of the tagged proteins meaning that changes in yeast resistance were certainly related to GFP ROS-scavenging ability (Yang).
Identifiants
pubmed: 36459911
pii: S1011-1344(22)00217-2
doi: 10.1016/j.jphotobiol.2022.112603
pii:
doi:
Substances chimiques
Green Fluorescent Proteins
147336-22-9
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112603Informations de copyright
Copyright © 2022 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors of the manuscript JPHOTOBIOL-D-22-00624 declare no conflict or competing interests. The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr. Stephane Guyot reports financial support was provided by Regional Council of Bourgogne Franche-Comté. Dr. Lucie Bertheau and Dr. Jennifer Dumont reports financial support was provided by Regional Council of Bourgogne Franche-Comté. Dr. Stephane Guyot reports financial support was provided by Fonds Européen de DEveloppement Régional (FEDER). Dr. Lucie Bertheau and Dr. Jennifer Dumont reports financial support was provided by Fonds Européen de DEveloppement Régional (FEDER). Dr. Stephane Guyot reports financial support was provided by AgroSup Dijon. Dr. Lucie Bertheau reports financial support was provided by AgroSup Dijon. Dr. Stephane Guyot reports financial support was provided by I-SITE Bourgogne Franche-Comté and MP2 master program.