A human
Human neo-cartilage
Mechanical stress
OA risk genes
Osteoarthritis
Journal
Osteoarthritis and cartilage open
ISSN: 2665-9131
Titre abrégé: Osteoarthr Cartil Open
Pays: England
ID NLM: 101767068
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
28
09
2021
revised:
06
12
2021
accepted:
20
12
2021
entrez:
7
12
2022
pubmed:
8
12
2022
medline:
8
12
2022
Statut:
epublish
Résumé
Due to the complexity and heterogeneity of osteoarthritis (OA) pathophysiology, studying the interaction between intrinsic molecular changes in chondrocytes after hyper-physiological mechanical stress (MS) and aberrant signalling of OA risk genes remains a challenge. In this study we set out to set up an Human primary chondrocyte neo-cartilage pellets were exposed for 2 days to 2 × 10 min of hyper-physiological dynamic MS attained by a 20% strain and a frequency of 5 Hz. In order to assess cartilage damage, sulphated glycosaminoglycan (sGAG) content in the neo-cartilage was quantified using Alcian blue staining and a dimethyl methylene blue (DMMB) assay, while cleavage of aggrecan was visualized by immunohistochemical staining of aggrecan neo-epitope NITEGE. In addition, changes in expression levels of catabolic, anabolic and hypertrophic genes, and of three OA risk genes; Hyper-physiological MS induced cartilage damage, as reflected by decreased sGAG content. mRNA levels of aggrecanase We established a human
Identifiants
pubmed: 36474468
doi: 10.1016/j.ocarto.2021.100231
pii: S2665-9131(21)00094-7
pmc: PMC9718246
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100231Informations de copyright
© 2021 The Authors.
Déclaration de conflit d'intérêts
All authors declare that they have nothing to disclose.
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