The ubiquitin-like protein Hub1/UBL-5 functions in pre-mRNA splicing in Caenorhabditis elegans.
Animals
RNA Precursors
/ genetics
Caenorhabditis elegans
/ genetics
Ubiquitins
/ metabolism
Saccharomyces cerevisiae
/ metabolism
RNA Splicing
Schizosaccharomyces
/ genetics
Mammals
/ genetics
Ligases
/ metabolism
Saccharomyces cerevisiae Proteins
/ metabolism
Caenorhabditis elegans Proteins
/ genetics
outron-containing transcripts
pre-mRNA splicing
spliceosome
trans-splicing and C. elegans
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
02 2023
02 2023
Historique:
revised:
25
11
2022
received:
24
03
2022
accepted:
28
11
2022
pubmed:
9
12
2022
medline:
16
2
2023
entrez:
8
12
2022
Statut:
ppublish
Résumé
The ubiquitin-like protein Hub1/UBL-5 associates with proteins non-covalently. Hub1 promotes alternative splicing and splicing of precursor mRNAs with weak introns in yeast and mammalian cells; however, its splicing function has remained elusive in multicellular organisms. Here, we demonstrate the splicing function of Hub1/UBL-5 in the free-living nematode Caenorhabditis elegans. Hub1/UBL-5 binds to the HIND-containing splicing factors Snu66/SART-1 and PRP-38 and associates with other spliceosomal proteins. C. elegans hub1/ubl-5 mutants die at the Larval 3 stage and show splicing defects for selected targets, similar to the mutants in yeast and mammalian cells. UBL-5 complemented growth and splicing defects in Schizosaccharomyces pombe hub1 mutants, confirming its functional conservation. Thus, UBL-5 is important for C. elegans development and plays a conserved pre-mRNA splicing function.
Identifiants
pubmed: 36480405
doi: 10.1002/1873-3468.14555
doi:
Substances chimiques
RNA Precursors
0
Ubiquitins
0
HUB1 protein, S cerevisiae
EC 6.3.2.-
Ligases
EC 6.-
Saccharomyces cerevisiae Proteins
0
Ubl-5 protein, C elegans
0
Caenorhabditis elegans Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
448-457Subventions
Organisme : NIH HHS
ID : P40 OD010440
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
© 2022 Federation of European Biochemical Societies.
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