Production of medium-chain-length PHA in octanoate-fed enrichments dominated by Sphaerotilus sp.
Sphaerotilus sp
medium-chain-length polyhydroxyalkanoates
microbial enrichment cultures
octanoate
poly(3-hydroxyhexanoate)
poly(3-hydroxyoctanoate)
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
revised:
01
12
2022
received:
22
06
2022
accepted:
12
12
2022
pubmed:
15
12
2022
medline:
16
2
2023
entrez:
14
12
2022
Statut:
ppublish
Résumé
Medium-chain-length polyhydroxyalkanoate (mcl-PHA) production by using microbial enrichments is a promising but largely unexplored approach to obtain elastomeric biomaterials from secondary resources. In this study, several enrichment strategies were tested to select a community with a high mcl-PHA storage capacity when feeding octanoate. On the basis of analysis of the metabolic pathways, the hypothesis was formulated that mcl-PHA production is more favorable under oxygen-limited conditions than short-chain-length PHA (scl-PHA). This hypothesis was confirmed by bioreactor experiments showing that oxygen limitation during the PHA accumulation experiments resulted in a higher fraction of mcl-PHA over scl-PHA (i.e., a PHA content of 76 wt% with an mcl fraction of 0.79 with oxygen limitation, compared to a PHA content of 72 wt% with an mcl-fraction of 0.62 without oxygen limitation). Physicochemical analysis revealed that the extracted PHA could be separated efficiently into a hydroxybutyrate-rich fraction with a higher M
Substances chimiques
octanoic acid
OBL58JN025
Caprylates
0
Polyhydroxyalkanoates
0
Hydroxybutyrates
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
687-701Subventions
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Informations de copyright
© 2022 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.
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