Lignin valorization to bioplastics with an aromatic hub metabolite-based autoregulation system.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 Oct 2024
28 Oct 2024
Historique:
received:
01
03
2024
accepted:
16
10
2024
medline:
29
10
2024
pubmed:
29
10
2024
entrez:
29
10
2024
Statut:
epublish
Résumé
Exploring microorganisms with downstream synthetic advantages in lignin valorization is an effective strategy to increase target product diversity and yield. This study ingeniously engineers the non-lignin-degrading bacterium Ralstonia eutropha H16 (also known as Cupriavidus necator H16) to convert lignin, a typically underutilized by-product of biorefinery, into valuable bioplastic polyhydroxybutyrate (PHB). The aromatic metabolism capacities of R. eutropha H16 for different lignin-derived aromatics (LDAs) are systematically characterized and complemented by integrating robust functional modules including O-demethylation, aromatic aldehyde metabolism and the mitigation of by-product inhibition. A pivotal discovery is the regulatory element PcaQ, which is highly responsive to the aromatic hub metabolite protocatechuic acid during lignin degradation. Based on the computer-aided design of PcaQ, we develop a hub metabolite-based autoregulation (HMA) system. This system can control the functional genes expression in response to heterologous LDAs and enhance metabolism efficiency. Multi-module genome integration and directed evolution further fortify the strain's stability and lignin conversion capacities, leading to PHB production titer of 2.38 g/L using heterologous LDAs as sole carbon source. This work not only marks a leap in bioplastic production from lignin components but also provides a strategy to redesign the non-LDAs-degrading microbes for efficient lignin valorization.
Identifiants
pubmed: 39468081
doi: 10.1038/s41467-024-53609-3
pii: 10.1038/s41467-024-53609-3
doi:
Substances chimiques
Lignin
9005-53-2
protocatechuic acid
36R5QJ8L4B
Hydroxybenzoates
0
Hydroxybutyrates
0
Polyesters
0
Biodegradable Plastics
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9288Subventions
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : Nos. 32170122
Informations de copyright
© 2024. The Author(s).
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