Defined Microenvironments Trigger In Vitro Gastrulation in Human Pluripotent Stem Cells.
embryogenesis
gastrulation
hydrogel
micropatterning
pluripotent stem cells
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
02 2023
02 2023
Historique:
revised:
19
09
2022
received:
22
06
2022
pubmed:
16
12
2022
medline:
17
2
2023
entrez:
15
12
2022
Statut:
ppublish
Résumé
Gastrulation is a stage in embryo development where three germ layers arise to dictate the human body plan. In vitro models of gastrulation have been demonstrated by treating pluripotent stem cells with soluble morphogens to trigger differentiation. However, in vivo gastrulation is a multistage process coordinated through feedback between soluble gradients and biophysical forces, with the multipotent epiblast transforming to the primitive streak followed by germ layer segregation. Here, the authors show how constraining pluripotent stem cells to hydrogel islands triggers morphogenesis that mirrors the stages preceding in vivo gastrulation, without the need for exogenous supplements. Within hours of initial seeding, cells display a contractile phenotype at the boundary, which leads to enhanced proliferation, yes-associated protein (YAP) translocation, epithelial to mesenchymal transition, and emergence of SRY-box transcription factor 17 (SOX17)
Identifiants
pubmed: 36519269
doi: 10.1002/advs.202203614
pmc: PMC9929265
doi:
Substances chimiques
YAP-Signaling Proteins
0
SOX17 protein, human
0
SOXF Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2203614Subventions
Organisme : Australian Research Council
ID : FT180100417
Organisme : National Health and Medical Research Council
ID : APP1185021
Organisme : NCI NIH HHS
Pays : United States
Organisme : NIH HHS
ID : R01CA251443
Pays : United States
Organisme : NIH HHS
ID : R01CA251443
Pays : United States
Informations de copyright
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.
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