Aligned silver nanowires for plasmonically-enhanced fluorescence detection of photoactive proteins in wet and dry environment.
Alignment
Fluorescence
Microchannel
Plasmon enhancement
Real-time detection
Silver nanowires
Journal
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
ISSN: 1873-3557
Titre abrégé: Spectrochim Acta A Mol Biomol Spectrosc
Pays: England
ID NLM: 9602533
Informations de publication
Date de publication:
15 Mar 2023
15 Mar 2023
Historique:
received:
29
04
2022
revised:
26
10
2022
accepted:
04
12
2022
pubmed:
16
12
2022
medline:
18
1
2023
entrez:
15
12
2022
Statut:
ppublish
Résumé
We developed a method of aligning silver nanowires in a microchannel and fixing them to glass substrates via appropriate functionalization. The attachment of nanowires to the substrate is robust with no variation of their angles over minutes. Specific conjugation with photoactive proteins is observed using wide-field fluorescence imaging in real-time for highly concentrated protein solution, both in a microchannel and in a chip geometry. In the latter case we can detect the presence of the proteins in the dropcasted solution down to single proteins. The results point towards possible implementation of aligned silver nanowires as geometrically defined plasmonic fluorescence sensing platforms.
Identifiants
pubmed: 36521338
pii: S1386-1425(22)01373-7
doi: 10.1016/j.saa.2022.122225
pii:
doi:
Substances chimiques
Silver
3M4G523W1G
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
122225Informations de copyright
Copyright © 2022 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.