Flow cytometry: a tool for understanding the behaviour of polyhydroxyalkanoate accumulators.
Bioreactors
Flow cytometry
Mixed microbial cultures
Polyhydroxyalkanoates
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Feb 2023
Feb 2023
Historique:
received:
27
04
2022
accepted:
26
11
2022
revised:
24
11
2022
pubmed:
17
12
2022
medline:
19
1
2023
entrez:
16
12
2022
Statut:
ppublish
Résumé
The use of mixed microbial cultures (MMCs) is seen as an attractive strategy for polyhydroxyalkanoate (PHA) production. In order to optimize the MMC-PHA production process, tools are required to improve our understanding of the physiological state of the PHA-storing microorganisms within the MMC. In the present study, we explored the use of flow cytometry to analyse the metabolic state and polyhydroxybutyrate (PHB) content of the microorganisms from an MMC-PHA production process. A sequencing batch reactor under a feast and famine regime was used to enrich an MMC with PHB-storing microorganisms. Interestingly, once the PHB-storing microorganisms are selected, the level of PHB accumulation depends largely on the metabolic state of these microorganisms and not exclusively on the consortium composition. These results demonstrate that flow cytometry is a powerful tool to help to understand the PHA storage response of an MMC-PHA production process. KEY POINTS: • Flow cytometry allows to measure PHB content and metabolic activity over time. • Microorganisms showing high PHB content also have high metabolic activity. • PHB producers with low metabolic activity show low PHB content.
Identifiants
pubmed: 36525042
doi: 10.1007/s00253-022-12318-x
pii: 10.1007/s00253-022-12318-x
doi:
Substances chimiques
Polyhydroxyalkanoates
0
polyhydroxybutyrate
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
581-590Subventions
Organisme : Agencia Nacional de Investigación y Desarrollo
ID : Anillo de Investigación en Ciencia y Tecnología GAMBIO Project N° ACT172128
Organisme : Agencia Nacional de Investigación y Desarrollo
ID : projecr for docrtoral thesis 21191476
Organisme : Agencia Nacional de Investigación y Desarrollo
ID : Postdoctoral Fondecyt 3200748
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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