Removal of sperm tail using trypsin and pre-activation of oocyte facilitates intracytoplasmic sperm injection in mice and rats.
Intracytoplasmic sperm injection (ICSI)
Mouse
Oocyte activation
Rat
Trypsin treatment
Journal
The Journal of reproduction and development
ISSN: 1348-4400
Titre abrégé: J Reprod Dev
Pays: Japan
ID NLM: 9438792
Informations de publication
Date de publication:
08 Feb 2023
08 Feb 2023
Historique:
pubmed:
19
12
2022
medline:
11
2
2023
entrez:
18
12
2022
Statut:
ppublish
Résumé
We examined various methods to enhance the accessibility of intracytoplasmic sperm injection (ICSI) technology to more users by making the technique easier, more efficient, and practical. First, the methods for artificially removing the mouse sperm tail were evaluated. Trypsin treatment was found to efficiently remove the sperm tails. The resultant sperm cells had a lower oocyte activation capacity; however, the use of activated oocytes resulted in the same fecundity as that of fresh, untreated sperm. Pre-activated oocytes were more resistant to physical damage, showed higher survival rates, and required less time per injection. Testing this method in rats yielded similar results, although the oocyte activation method was different. Remarkably, this method resulted in higher birth rates of rat progeny than with conventional methods of rat ICSI. Our method thereby streamlines mouse and rat ICSI, making it more accessible to laboratories across many disciplines.
Identifiants
pubmed: 36529517
doi: 10.1262/jrd.2022-065
pmc: PMC9939287
doi:
Substances chimiques
Trypsin
EC 3.4.21.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
48-52Références
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