Activity, Template Preference, and Compatibility of Components of RNA Replicase of Eastern Equine Encephalitis Virus.


Journal

Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724

Informations de publication

Date de publication:
31 01 2023
Historique:
pubmed: 20 12 2022
medline: 3 2 2023
entrez: 19 12 2022
Statut: ppublish

Résumé

Eastern equine encephalitis virus (EEEV) usually cycles between Culiseta melanura mosquitoes and birds; however, it can also infect humans. EEEV has a positive-sense RNA genome that, in infected cells, serves as an mRNA for the P1234 polyprotein. P1234 undergoes a series of precise cleavage events producing four nonstructural proteins (nsP1-4) representing subunits of the RNA replicase. Here, we report the construction and properties of a

Identifiants

pubmed: 36533950
doi: 10.1128/jvi.01368-22
pmc: PMC9888243
doi:

Substances chimiques

RNA, Viral 0
RNA-Dependent RNA Polymerase EC 2.7.7.48
Viral Nonstructural Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0136822

Subventions

Organisme : Medical Research Council
ID : MC_PC_16028
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 200171/Z/15/Z
Pays : United Kingdom

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Auteurs

Laura Sandra Lello (LS)

Institute of Technology, University of Tartu, Tartu, Estonia.

Agneta Miilimäe (A)

Institute of Technology, University of Tartu, Tartu, Estonia.

Liubov Cherkashchenko (L)

Institute of Technology, University of Tartu, Tartu, Estonia.

Ailar Omler (A)

Institute of Technology, University of Tartu, Tartu, Estonia.

Rachel Skilton (R)

CBR Division, Dstl Porton Down, Salisbury, UK.

Rachel Ireland (R)

CBR Division, Dstl Porton Down, Salisbury, UK.

David Ulaeto (D)

CBR Division, Dstl Porton Down, Salisbury, UK.

Andres Merits (A)

Institute of Technology, University of Tartu, Tartu, Estonia.

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Classifications MeSH