Multivalency ensures persistence of a +TIP body at specialized microtubule ends.
Journal
Nature cell biology
ISSN: 1476-4679
Titre abrégé: Nat Cell Biol
Pays: England
ID NLM: 100890575
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
received:
14
10
2021
accepted:
21
10
2022
pubmed:
20
12
2022
medline:
25
1
2023
entrez:
19
12
2022
Statut:
ppublish
Résumé
Microtubule plus-end tracking proteins (+TIPs) control microtubule specialization and are as such essential for cell division and morphogenesis. Here we investigated interactions and functions of the budding yeast Kar9 network consisting of the core +TIP proteins Kar9 (functional homologue of APC, MACF and SLAIN), Bim1 (orthologous to EB1) and Bik1 (orthologous to CLIP-170). A multivalent web of redundant interactions links the three +TIPs together to form a '+TIP body' at the end of chosen microtubules. This body behaves as a liquid condensate that allows it to persist on both growing and shrinking microtubule ends, and to function as a mechanical coupling device between microtubules and actin cables. Our study identifies nanometre-scale condensates as effective cellular structures and underlines the power of dissecting the web of low-affinity interactions driving liquid-liquid phase separation in order to establish how condensation processes support cell function.
Identifiants
pubmed: 36536177
doi: 10.1038/s41556-022-01035-2
pii: 10.1038/s41556-022-01035-2
pmc: PMC9859758
doi:
Substances chimiques
Microtubule-Associated Proteins
0
KAR1 protein, S cerevisiae
0
BIM1 protein, S cerevisiae
0
Bik1 protein, S cerevisiae
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
56-67Subventions
Organisme : EC | EC Seventh Framework Programm | FP7 People: Marie-Curie Actions (FP7-PEOPLE - Specific Programme "People" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013))
ID : 290605
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)
ID : RTD Grant #2012/192 TubeX
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)
ID : CRSII5_189940
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)
ID : 310030_192566
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)
ID : 31003A-105904
Informations de copyright
© 2022. The Author(s).
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