A Compact Control System to Enable Automated Operation of Microfluidic Bioanalytical Assays.


Journal

Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191

Informations de publication

Date de publication:
13 Dec 2022
Historique:
received: 07 09 2022
revised: 31 10 2022
accepted: 01 11 2022
entrez: 23 12 2022
pubmed: 24 12 2022
medline: 27 12 2022
Statut: epublish

Résumé

We describe a control system for operating valve-enabled microfluidic devices and leverage this control system to carry out a complex workflow of plasma separation from 8 μL of whole blood followed by on-chip mixing of plasma with assay reagents for biomarker detection. The control system incorporates pumps, digital pressure sensors, a microcontroller, solenoid valves and off-the-shelf components to deliver high and low air pressure in the desired temporal sequence to meter fluid flow and actuate microvalves. Importantly, our control system is portable, which is suitable for operating the microvalve-enabled microfluidic devices in the point-of-care setting.

Identifiants

pubmed: 36551127
pii: bios12121160
doi: 10.3390/bios12121160
pmc: PMC9775492
pii:
doi:

Substances chimiques

Biomarkers 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIDDK NIH HHS
ID : P30 DK084567
Pays : United States
Organisme : NICHD NIH HHS
ID : R41 HD100251
Pays : United States
Organisme : NIH HHS
ID : HD100251
Pays : United States
Organisme : NIH HHS
ID : DK084567
Pays : United States

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Auteurs

Alan M Gonzalez-Suarez (AM)

Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA.

Alexander Long (A)

Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA.
Department of Biology, St. Olaf College, Northfield, MN 55057, USA.

XuHai Huang (X)

Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA.

Alexander Revzin (A)

Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 55905, USA.

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Classifications MeSH