Nerve Targeting via Myelin Protein Zero and the Impact of Dimerization on Binding Affinity.
fluorescence imaging
fluorescence-guided surgery
myelin protein zero
nerve targeting
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
17 Dec 2022
17 Dec 2022
Historique:
received:
03
11
2022
revised:
07
12
2022
accepted:
13
12
2022
entrez:
23
12
2022
pubmed:
24
12
2022
medline:
27
12
2022
Statut:
epublish
Résumé
Surgically induced nerve damage is a common but debilitating side effect. By developing tracers that specifically target the most abundant protein in peripheral myelin, namely myelin protein zero (P0), we intend to support fluorescence-guided nerve-sparing surgery. To that end, we aimed to develop a dimeric tracer that shows a superior affinity for P0. Following truncation of homotypic P0 protein-based peptide sequences and fluorescence labeling, the lead compound Cy5-P0 Dimerization resulted in a 1.5-fold increase in affinity compared to the mono-labeled counterpart (70.3 +/- 10.0 nM vs. 104.9 +/- 16.7 nM; Dimerization of the nerve-targeting peptide P0
Sections du résumé
BACKGROUND
BACKGROUND
Surgically induced nerve damage is a common but debilitating side effect. By developing tracers that specifically target the most abundant protein in peripheral myelin, namely myelin protein zero (P0), we intend to support fluorescence-guided nerve-sparing surgery. To that end, we aimed to develop a dimeric tracer that shows a superior affinity for P0.
METHODS
METHODS
Following truncation of homotypic P0 protein-based peptide sequences and fluorescence labeling, the lead compound Cy5-P0
RESULTS
RESULTS
Dimerization resulted in a 1.5-fold increase in affinity compared to the mono-labeled counterpart (70.3 +/- 10.0 nM vs. 104.9 +/- 16.7 nM;
CONCLUSIONS
CONCLUSIONS
Dimerization of the nerve-targeting peptide P0
Identifiants
pubmed: 36558148
pii: molecules27249015
doi: 10.3390/molecules27249015
pmc: PMC9786614
pii:
doi:
Substances chimiques
Myelin P0 Protein
0
cyanine dye 5
0
Peptides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Resuscitation Council
ID : FWO S000922N
Organisme : NWO-TTW-VICI
ID : TTW BGT16141
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