Detection of lisdexamfetamine and its metabolite d-amphetamine in urine and gastric contents collected from a cadaver at forensic autopsy.
Amphetamine
Autopsy samples
LC–MS/MS
Lisdexamfetamine (LDX)
Optical isomer separation
Stability of LDX in whole blood
Journal
Forensic toxicology
ISSN: 1860-8965
Titre abrégé: Forensic Toxicol
Pays: Japan
ID NLM: 101315563
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
received:
06
04
2022
accepted:
12
12
2022
medline:
3
7
2023
pubmed:
24
12
2022
entrez:
23
12
2022
Statut:
ppublish
Résumé
Lisdexamfetamine (LDX), which is used for the treatment of attention-deficit/hyperactivity disorder and narcolepsy, is composed of L-lysine attached to dextroamphetamine (d-amphetamine). In this article, we report a forensic autopsy case in which prescription drugs were unknown at autopsy. While amphetamine was detected, methamphetamine could not be detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in any of samples collected. Thus, we aimed to quantify LDX concentrations in autopsy samples and to prove that the amphetamine detected in this case was due to metabolized LDX. Femoral vein blood, cardiac whole blood, urine, and gastric content samples were taken at autopsy for toxicological analysis. Qualitative and quantitative analyses were performed using LC-MS/MS. In addition, optical isomer separation for the amphetamine detected was conducted. The stability of LDX in whole blood and urine was also examined at three different temperatures. The concentrations of LDX were < 4.00, 30.9, and 4.42 ng/mL in whole blood, urine, and gastric content samples, respectively. The concentrations of amphetamine were 329, 510, 2970, and 915 ng/mL in femoral vein blood, heart whole blood, urine, and gastric contents, respectively. The amphetamine detected in this case was identified to be only d-amphetamine by optical isomer separation. The d-amphetamine detected was considered to be derived from LDX. Stability experiments revealed that LDX in whole blood decreased at ambient temperature. The results in the present case report may be useful in interpreting whether or not the amphetamine detected in a cadaver is a metabolite of LDX.
Identifiants
pubmed: 36562920
doi: 10.1007/s11419-022-00654-6
pii: 10.1007/s11419-022-00654-6
pmc: PMC10310599
doi:
Substances chimiques
Lisdexamfetamine Dimesylate
SJT761GEGS
Amphetamine
CK833KGX7E
Dextroamphetamine
TZ47U051FI
Prodrugs
0
Types de publication
Case Reports
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
309-317Informations de copyright
© 2022. The Author(s).
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