Localization and potential role of prostate microbiota.
beneign prostate hyperplasia
benign prostate enlargement
in situ hybridization
microbiome
microbiota
prostate
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2022
2022
Historique:
received:
19
09
2022
accepted:
09
11
2022
entrez:
26
12
2022
pubmed:
27
12
2022
medline:
28
12
2022
Statut:
epublish
Résumé
We aimed to clarify the presence and localization of the prostate microbiota and examine its association with benign prostate enlargement (BPE). The microbiota of prostate tissues and catheterized urine from 15 patients were analyzed by 16S metagenomic analysis and compared to show that the prostate microbiota was not a contaminant of the urinary microbiota. Fluorescence in situ hybridization (FISH) and in situ hybridization (ISH) using the specific probe for eubacteria was performed on prostate tissue to show the localization of bacteria in the prostate. The BPE group was defined as prostate volume ≥30 mL, and the non-BPE group as prostate volume <30 mL. The microbiota of the two groups were compared to clarify the association between prostate microbiota and BPE. Faith's phylogenetic diversity index of prostate tissue was significantly higher than that of urine (42.3±3.8 vs 25.5±5.6, P=0.01). Principal coordinate analysis showed a significant difference between the microbiota of prostate tissue and catheterized urine (P<0.01). FISH and ISH showed the presence of bacteria in the prostatic duct. Comparison of prostate microbiota between the BPE and non-BPE groups showed that the Chao1 index of the BPE group was significantly lower than that of the latter [142 (50-316) vs 169 (97-665), P=0.047] and the abundance of Burkholderia was significantly higher in the BPE group than in the latter. We demonstrated that the prostate microbiota was located in the prostatic duct and reduced diversity of prostate microbiota was associated with BPE, suggesting that prostate microbiota plays a role in BPE.
Identifiants
pubmed: 36569206
doi: 10.3389/fcimb.2022.1048319
pmc: PMC9768196
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1048319Informations de copyright
Copyright © 2022 Okada, Takezawa, Tsujimura, Imanaka, Kuribayashi, Ueda, Hatano, Fukuhara, Kiuchi, Fujita, Motooka, Nakamura, Koyama, Shimada and Nonomura.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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