Implantable niche with local immunosuppression for islet allotransplantation achieves type 1 diabetes reversal in rats.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
26 12 2022
Historique:
received: 22 01 2022
accepted: 14 12 2022
entrez: 26 12 2022
pubmed: 27 12 2022
medline: 29 12 2022
Statut: epublish

Résumé

Pancreatic islet transplantation efficacy for type 1 diabetes (T1D) management is limited by hypoxia-related graft attrition and need for systemic immunosuppression. To overcome these challenges, we developed the Neovascularized Implantable Cell Homing and Encapsulation (NICHE) device, which integrates direct vascularization for facile mass transfer and localized immunosuppressant delivery for islet rejection prophylaxis. Here, we investigated NICHE efficacy for allogeneic islet transplantation and long-term diabetes reversal in an immunocompetent, male rat model. We demonstrated that allogeneic islets transplanted within pre-vascularized NICHE were engrafted, revascularized, and functional, reverting diabetes in rats for over 150 days. Notably, we confirmed that localized immunosuppression prevented islet rejection without inducing toxicity or systemic immunosuppression. Moreover, for translatability efforts, we showed NICHE biocompatibility and feasibility of deployment as well as short-term allogeneic islet engraftment in an MHC-mismatched nonhuman primate model. In sum, the NICHE holds promise as a viable approach for safe and effective islet transplantation and long-term T1D management.

Identifiants

pubmed: 36572684
doi: 10.1038/s41467-022-35629-z
pii: 10.1038/s41467-022-35629-z
pmc: PMC9792517
doi:

Substances chimiques

Immunosuppressive Agents 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

7951

Subventions

Organisme : NIDDK NIH HHS
ID : R01 DK132104
Pays : United States

Informations de copyright

© 2022. The Author(s).

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Auteurs

Jesus Paez-Mayorga (J)

Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA.
School of Medicine and Health Sciences, Tecnologico de Monterrey, Monterrey, NL, Mexico.

Jocelyn Nikita Campa-Carranza (JN)

Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA.
School of Medicine and Health Sciences, Tecnologico de Monterrey, Monterrey, NL, Mexico.

Simone Capuani (S)

Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA.
University of the Chinese Academy of Sciences (UCAS), Shijingshan, Beijing, China.

Nathanael Hernandez (N)

Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA.

Hsuan-Chen Liu (HC)

Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA.

Corrine Ying Xuan Chua (CYX)

Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA.

Fernanda Paola Pons-Faudoa (FP)

Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA.

Gulsah Malgir (G)

Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA.

Bella Alvarez (B)

Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA.
School of Medicine and Health Sciences, Tecnologico de Monterrey, Monterrey, NL, Mexico.

Jean A Niles (JA)

Center for Tissue Engineering, Houston Methodist Research Institute, Houston, TX, USA.

Lissenya B Argueta (LB)

Center for Tissue Engineering, Houston Methodist Research Institute, Houston, TX, USA.

Kathryn A Shelton (KA)

Department of Comparative Medicine, Michael E. Keeling Center for Comparative Medicine and Research, MD Anderson Cancer Center, Bastrop, TX, USA.

Sarah Kezar (S)

Department of Comparative Medicine, Michael E. Keeling Center for Comparative Medicine and Research, MD Anderson Cancer Center, Bastrop, TX, USA.

Pramod N Nehete (PN)

Department of Comparative Medicine, Michael E. Keeling Center for Comparative Medicine and Research, MD Anderson Cancer Center, Bastrop, TX, USA.
The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX, USA.

Dora M Berman (DM)

Diabetes Research Institute, University of Miami, Miami, FL, USA.
Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL, USA.

Melissa A Willman (MA)

Diabetes Research Institute, University of Miami, Miami, FL, USA.

Xian C Li (XC)

Department of Surgery, Houston Methodist Hospital, Houston, TX, USA.
Immunobiology and Transplant Science Center, Houston Methodist Hospital, Houston, TX, USA.

Camillo Ricordi (C)

Diabetes Research Institute, University of Miami, Miami, FL, USA.

Joan E Nichols (JE)

Center for Tissue Engineering, Houston Methodist Research Institute, Houston, TX, USA.
Department of Surgery, Houston Methodist Hospital, Houston, TX, USA.

A Osama Gaber (AO)

Department of Surgery, Houston Methodist Hospital, Houston, TX, USA.

Norma S Kenyon (NS)

Diabetes Research Institute, University of Miami, Miami, FL, USA.
Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL, USA.
Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, Miami, FL, USA.
Department of Biomedical Engineering, University of Miami, Miami, FL, USA.
Department of Biochemistry and Molecular Biology, University of Miami, Miami, FL, USA.

Alessandro Grattoni (A)

Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA. agrattoni@houstonmethodist.org.
Department of Surgery, Houston Methodist Hospital, Houston, TX, USA. agrattoni@houstonmethodist.org.
Department of Biochemistry and Molecular Biology, University of Miami, Miami, FL, USA. agrattoni@houstonmethodist.org.
Department of Radiation Oncology, Houston Methodist Hospital, Houston, TX, USA. agrattoni@houstonmethodist.org.

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