High-frequency synthetic apomixis in hybrid rice.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
27 12 2022
27 12 2022
Historique:
received:
02
09
2022
accepted:
18
12
2022
entrez:
27
12
2022
pubmed:
28
12
2022
medline:
30
12
2022
Statut:
epublish
Résumé
Introducing asexual reproduction through seeds - apomixis - into crop species could revolutionize agriculture by allowing F1 hybrids with enhanced yield and stability to be clonally propagated. Engineering synthetic apomixis has proven feasible in inbred rice through the inactivation of three genes (MiMe), which results in the conversion of meiosis into mitosis in a line ectopically expressing the BABYBOOM1 (BBM1) parthenogenetic trigger in egg cells. However, only 10-30% of the seeds are clonal. Here, we show that synthetic apomixis can be achieved in an F1 hybrid of rice by inducing MiMe mutations and egg cell expression of BBM1 in a single step. We generate hybrid plants that produce more than 95% of clonal seeds across multiple generations. Clonal apomictic plants maintain the phenotype of the F1 hybrid along successive generations. Our results demonstrate that there is no barrier to almost fully penetrant synthetic apomixis in an important crop species, rendering it compatible with use in agriculture.
Identifiants
pubmed: 36575169
doi: 10.1038/s41467-022-35679-3
pii: 10.1038/s41467-022-35679-3
pmc: PMC9794695
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7963Informations de copyright
© 2022. The Author(s).
Références
Transgenic Res. 1996 May;5(3):213-8
pubmed: 8673150
Annu Rev Plant Biol. 2013;64:71-88
pubmed: 23394499
PLoS Genet. 2012;8(7):e1002865
pubmed: 22844260
Stain Technol. 1969 May;44(3):117-22
pubmed: 4181665
Plant Physiol. 2011 Feb;155(2):881-91
pubmed: 21106719
Science. 2011 Feb 18;331(6019):876
pubmed: 21330535
PLoS Biol. 2009 Jun 9;7(6):e1000124
pubmed: 19513101
Theor Appl Genet. 1997 Jan;94(1):27-33
pubmed: 19352741
Carbohydr Polym. 2013 Oct 15;98(1):650-8
pubmed: 23987395
Bioinformatics. 2009 Jul 15;25(14):1754-60
pubmed: 19451168
Plant Cell Rep. 2005 Mar;23(10-11):759-63
pubmed: 15459795
Rice (N Y). 2014 Dec;7(1):13
pubmed: 26055995
Theor Appl Genet. 2003 May;106(8):1396-408
pubmed: 12677401
Genetics. 2014 Jun;197(2):441-50
pubmed: 24939990
PLoS Genet. 2018 Mar 7;14(3):e1007230
pubmed: 29513662
Genome Biol. 2015 Oct 01;16:217
pubmed: 26429765
Dev Cell. 2019 Jun 17;49(6):882-893.e5
pubmed: 31080059
Plant Commun. 2020 Jul 02;2(1):100092
pubmed: 33511344
Plant Cell. 2004 Apr;16(4):1008-20
pubmed: 15031413
Plant Physiol. 2018 Feb;176(2):1627-1645
pubmed: 29196538
Plant J. 2007 Jul;51(2):281-92
pubmed: 17559508
Cell Res. 2016 Nov;26(11):1242-1254
pubmed: 27767093
Nucleic Acids Res. 2007 Jan;35(Database issue):D883-7
pubmed: 17145706
Curr Opin Plant Biol. 2021 Feb;59:101993
pubmed: 33422964
Science. 2012 Nov 23;338(6110):1093-7
pubmed: 23180860
Trends Genet. 2020 Mar;36(3):215-226
pubmed: 31973878
Nucleic Acids Res. 2014;42(17):10903-14
pubmed: 25200087
Philos Trans R Soc Lond B Biol Sci. 2021 Jun 7;376(1826):20200118
pubmed: 33866810
Cell Res. 2013 Oct;23(10):1233-6
pubmed: 23999856
Yi Chuan Xue Bao. 2002;29(11):1001-4
pubmed: 12645264
Nat Genet. 2013 May;45(5):573-7
pubmed: 23502780
Nucleic Acids Res. 1999 Jan 15;27(2):573-80
pubmed: 9862982
EMBO J. 2001 Feb 1;20(3):589-600
pubmed: 11157765
Science. 1990 Nov 16;250(4983):942-7
pubmed: 17746917
Nat Rev Genet. 2013 Jul;14(7):471-82
pubmed: 23752794
Nat Commun. 2017 Oct 23;8(1):991
pubmed: 29062086
Nat Plants. 2015 Jul 06;1:15092
pubmed: 27250257
PLoS Genet. 2016 Feb 12;12(2):e1005856
pubmed: 26871453
Annu Rev Plant Biol. 2022 May 20;73:201-225
pubmed: 35138881
Nature. 2010 Mar 25;464(7288):615-8
pubmed: 20336146
Genomics Proteomics Bioinformatics. 2022 Jun;20(3):524-535
pubmed: 33711466
Nucleic Acids Res. 2012 Jan;40(Database issue):D1178-86
pubmed: 22110026
Dev Cell. 2017 Nov 6;43(3):349-358.e4
pubmed: 29112853
Plant Cell. 1999 Mar;11(3):417-30
pubmed: 10072401
Nat Plants. 2018 Aug;4(8):530-533
pubmed: 29988153
Curr Biol. 2016 Feb 8;26(3):331-7
pubmed: 26832437
Plant Reprod. 2017 Mar;30(1):41-52
pubmed: 28238020
Plant Physiol. 2011 Jul;156(3):1386-96
pubmed: 21606318
PLoS Genet. 2010 Jun 17;6(6):e1000989
pubmed: 20585549
Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):11205-10
pubmed: 26305939
Nature. 2019 Jan;565(7737):91-95
pubmed: 30542157
Planta. 2001 Jul;213(3):370-8
pubmed: 11506359
Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):10007-12
pubmed: 24958856
J Integr Plant Biol. 2020 Mar;62(3):287-298
pubmed: 30916464
Nat Biotechnol. 2019 Mar;37(3):283-286
pubmed: 30610223
Annu Rev Genet. 2007;41:509-37
pubmed: 18076331