Steering Stem Cell Fate within 3D Living Composite Tissues Using Stimuli-Responsive Cell-Adhesive Micromaterials.
3D cell culture
cell-matrix interactions
microgels
smart materials
tissue engineering
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
revised:
28
11
2022
received:
21
09
2022
medline:
6
4
2023
pubmed:
5
1
2023
entrez:
4
1
2023
Statut:
ppublish
Résumé
Engineered living microtissues such as cellular spheroids and organoids have enormous potential for the study and regeneration of tissues and organs. Microtissues are typically engineered via self-assembly of adherent cells into cellular spheroids, which are characterized by little to no cell-material interactions. Consequently, 3D microtissue models currently lack structural biomechanical and biochemical control over their internal microenvironment resulting in suboptimal functional performance such as limited stem cell differentiation potential. Here, this work report on stimuli-responsive cell-adhesive micromaterials (SCMs) that can self-assemble with cells into 3D living composite microtissues through integrin binding, even under serum-free conditions. It is demonstrated that SCMs homogeneously distribute within engineered microtissues and act as biomechanically and biochemically tunable designer materials that can alter the composite tissue microenvironment on demand. Specifically, cell behavior is controlled based on the size, stiffness, number ratio, and biofunctionalization of SCMs in a temporal manner via orthogonal secondary crosslinking strategies. Photo-based mechanical tuning of SCMs reveals early onset stiffness-controlled lineage commitment of differentiating stem cell spheroids. In contrast to conventional encapsulation of stem cell spheroids within bulk hydrogel, incorporating cell-sized SCMs within stem cell spheroids uniquely provides biomechanical cues throughout the composite microtissues' volume, which is demonstrated to be essential for osteogenic differentiation.
Identifiants
pubmed: 36599686
doi: 10.1002/advs.202205487
pmc: PMC10074101
doi:
Substances chimiques
Hydrogels
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2205487Subventions
Organisme : European Fund for Regional Development
ID : #PROJ-00963
Organisme : Dutch Arthritis Foundation
ID : #12-2-411
Organisme : Dutch Arthritis Foundation
ID : #LLP-25
Organisme : NWO-Groot consortium
ID : OCENW.GROOT.2019.079
Organisme : Dutch Research Council
ID : #17522
Pays : Netherlands
Organisme : European Research Council
ID : #759425
Pays : International
Informations de copyright
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
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