Fasciola hepatica primoinfections and reinfections in sheep drive distinct Th1/Th2/Treg immune responses in liver and hepatic lymph node at early and late stages.
Fasciola hepatica
Th1
Th2
Treg
hepatic lymph nodes
immune response
liver
primoinfections
reinfections
vaccine
Journal
Veterinary research
ISSN: 1297-9716
Titre abrégé: Vet Res
Pays: England
ID NLM: 9309551
Informations de publication
Date de publication:
10 Jan 2023
10 Jan 2023
Historique:
received:
25
05
2022
accepted:
24
10
2022
entrez:
10
1
2023
pubmed:
11
1
2023
medline:
13
1
2023
Statut:
epublish
Résumé
The expression of proinflammatory (IL-1β, IFN-γ, TNF-α) and regulatory (IL-10, TGF-β, IL-4) cytokines, as well as the transcription factor FoxP3, was quantified in the liver and hepatic lymph node (HLN) of sheep primoinfected and reinfected with Fasciola hepatica at early (4, 8 and 16 days post-infection [dpi]) and late (100 dpi) stages. The liver exerted a Th2 immune response at very early stages after the primoinfection with F. hepatica that induced the downregulation of IFN-γ, followed by a Th1/Th2/Treg response although the late stages were characterised by the expression of Th1/Th2 immune mediators. Contrarily, in reinfected sheep a robust mixed Th1/Th2/Treg immune response was found at very early stages meanwhile at late stages we observed a Th2/Treg immune response overcoming the expression of Th1 immune mediators. However, the HLN displayed a completely different Th1/Th2/Treg expression profile compared to the liver. Primoinfections with F. hepatica in HLN induced a mixed Th1/Th2/Treg environment from early stages, establishing a Th2 immune response at a late stage. However, the reinfected sheep exerted a Th2 immune response at early stages led by the IL-4 expression in opposition to the Th1/Th2/Treg found in the liver, meanwhile at late stages the HLN of reinfected sheep exerted a mixed Th1/Th2/Treg immune response. This is the first work publishing the expression of immune mediators in the liver and HLN from reinfected sheep with F. hepatica. The study of the immune responses exerted by the natural host in the target organs directly implied in the development of F. hepatica are crucial to better understand the immunopathogenesis of the fasciolosis being a key factor to develop effective vaccines.
Identifiants
pubmed: 36627694
doi: 10.1186/s13567-022-01129-7
pii: 10.1186/s13567-022-01129-7
pmc: PMC9832625
doi:
Substances chimiques
Interleukin-4
207137-56-2
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2Subventions
Organisme : Ministerio de Ciencia e Innovación
ID : PID2019-108782RB-C21
Organisme : Ministerio de Ciencia e Innovación
ID : AGL2015-67023-C2-2-R
Organisme : Consejería de Conocimiento, Investigación y Universidad, Junta de Andalucía
ID : P18-RTJ-1956
Informations de copyright
© 2023. The Author(s).
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