Bladder-colon chronic cross-sensitization involves neuro-glial pathways in male mice.
Animals
Male
Mice
Rats
CX3C Chemokine Receptor 1
/ metabolism
Green Fluorescent Proteins
Inflammation
/ metabolism
Mice, Inbred C57BL
Proto-Oncogene Proteins c-fos
/ metabolism
Rats, Sprague-Dawley
Spinal Cord
/ physiopathology
Urinary Bladder
/ innervation
Visceral Pain
/ physiopathology
Colon
/ innervation
Hyperalgesia
/ physiopathology
Chronic Pain
/ physiopathology
Microglia
/ physiology
Cross-organ sensitization
MAPK-p38
Microglia
NK1 receptor
Pain
Visceral hypersensitivity
Journal
World journal of gastroenterology
ISSN: 2219-2840
Titre abrégé: World J Gastroenterol
Pays: United States
ID NLM: 100883448
Informations de publication
Date de publication:
28 Dec 2022
28 Dec 2022
Historique:
received:
18
05
2022
revised:
02
10
2022
accepted:
26
10
2022
entrez:
12
1
2023
pubmed:
13
1
2023
medline:
14
1
2023
Statut:
ppublish
Résumé
Irritable bowel syndrome and bladder pain syndrome often overlap and are both characterized by visceral hypersensitivity. Since pelvic organs share common sensory pathways, it is likely that those syndromes involve a cross-sensitization of the bladder and the colon. The precise pathophysiology remains poorly understood. To develop a model of chronic bladder-colon cross-sensitization and to investigate the mech-anisms involved. Chronic cross-organ visceral sensitization was obtained in C57BL/6 mice using ultrasound-guided intravesical injections of acetic acid under brief isoflurane anesthesia. Colorectal sensitivity was assessed in conscious mice by measuring intracolonic pressure during isobaric colorectal distensions. Myeloperoxidase, used as a marker of colorectal inflammation, was measured in the colon, and colorectal permeability was measured using chambers. c-Fos protein expression, used as a marker of neuronal activation, was assessed in the spinal cord (L6-S1 level) using immunohistochemistry. Green fluorescent protein on the fractalkine receptor-positive mice were used to identify and count microglia cells in the L6-S1 dorsal horn of the spinal cord. The expression of NK1 receptors and MAPK-p38 were quantified in the spinal cord using western blot. Visceral hypersensitivity to colorectal distension was observed after the intravesical injection of acetic acid We describe a new model of cross-organ visceral sensitization between the bladder and the colon in mice. Intravesical injections of acetic acid induced a long-lasting colorectal hypersensitivity to distension, mediated by neuroglial interactions, MAPK-p38 phosphorylation and the NK1 receptor.
Sections du résumé
BACKGROUND
BACKGROUND
Irritable bowel syndrome and bladder pain syndrome often overlap and are both characterized by visceral hypersensitivity. Since pelvic organs share common sensory pathways, it is likely that those syndromes involve a cross-sensitization of the bladder and the colon. The precise pathophysiology remains poorly understood.
AIM
OBJECTIVE
To develop a model of chronic bladder-colon cross-sensitization and to investigate the mech-anisms involved.
METHODS
METHODS
Chronic cross-organ visceral sensitization was obtained in C57BL/6 mice using ultrasound-guided intravesical injections of acetic acid under brief isoflurane anesthesia. Colorectal sensitivity was assessed in conscious mice by measuring intracolonic pressure during isobaric colorectal distensions. Myeloperoxidase, used as a marker of colorectal inflammation, was measured in the colon, and colorectal permeability was measured using chambers. c-Fos protein expression, used as a marker of neuronal activation, was assessed in the spinal cord (L6-S1 level) using immunohistochemistry. Green fluorescent protein on the fractalkine receptor-positive mice were used to identify and count microglia cells in the L6-S1 dorsal horn of the spinal cord. The expression of NK1 receptors and MAPK-p38 were quantified in the spinal cord using western blot.
RESULTS
RESULTS
Visceral hypersensitivity to colorectal distension was observed after the intravesical injection of acetic acid
CONCLUSION
CONCLUSIONS
We describe a new model of cross-organ visceral sensitization between the bladder and the colon in mice. Intravesical injections of acetic acid induced a long-lasting colorectal hypersensitivity to distension, mediated by neuroglial interactions, MAPK-p38 phosphorylation and the NK1 receptor.
Identifiants
pubmed: 36632316
doi: 10.3748/wjg.v28.i48.6935
pmc: PMC9827584
doi:
Substances chimiques
CX3C Chemokine Receptor 1
0
Green Fluorescent Proteins
147336-22-9
Proto-Oncogene Proteins c-fos
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6935-6949Informations de copyright
©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict-of-interest statement: None of the authors have any conflicts of interest.
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