Biochemical characterization and mechanistic insight of the family IV uracil DNA glycosylase from Sulfolobus islandicus REY15A.

Uracil-DNA Glycosidase / chemistry Sulfolobus / genetics Amino Acid Sequence DNA / metabolism DNA Repair Archaea / metabolism Uracil / metabolism
DNA repair Deamination Hyperthermophilic Archaea Uracil DNA glycosylase

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
01 Mar 2023
Historique:
received: 25 07 2022
revised: 23 12 2022
accepted: 07 01 2023
pubmed: 14 1 2023
medline: 16 2 2023
entrez: 13 1 2023
Statut: ppublish

Résumé

Uracil DNA glycosylase (UDG) can remove uracil from DNA, thus playing an essential role in maintaining genomic stability. Family IV UDG members are mostly widespread in hyperthermophilic Archaea and bacteria. In this work, we characterized the family IV UDG from the hyperthermophilic crenarchaeon Sulfolobus islandicus REY15A (Sis-UDGIV) biochemically, and dissected the roles of nine conserved residues in uracil excision by mutational analyses. Biochemical data demonstrate that Sis-UDGIV displays maximum efficiency for uracil excision at 50 °C ~ 70 °C and at pH 7.0-9.0. Additionally, the enzyme has displays a weak activity without a divalent metal ion, but maximum activity with Mg

Identifiants

DOI: 10.1016/j.ijbiomac.2023.123222 PMID: 36639072
pubmed: 36639072
pii: S0141-8130(23)00098-3
doi: 10.1016/j.ijbiomac.2023.123222
pii:
doi:

Substances chimiques

Uracil-DNA Glycosidase EC 3.2.2.-
DNA 9007-49-2
Uracil 56HH86ZVCT

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

123222

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest All authors declare that there is no conflict of interests regarding the publication of this paper.

Auteurs

Mai Wu (M)

College of Environmental Science and Engineering, Yangzhou University, China.

Tan Lin (T)

College of Environmental Science and Engineering, Yangzhou University, China.

Kunming Dong (K)

College of Environmental Science and Engineering, Yangzhou University, China.

Yong Gong (Y)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China.

Xipeng Liu (X)

Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, China.

Likui Zhang (L)

College of Environmental Science and Engineering, Yangzhou University, China; Guangling College, Yangzhou University, China. Electronic address: lkzhang@yzu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Enzymes de réparation de l'ADN › Uracil-DNA glycosidase › Biochemical characterization and mechanistic...
questionsmedicales.fr › Archéobactéries › Crenarchaeota › Sulfolobale › Sulfolobaceae › Sulfolobus › Biochemical characterization and mechanistic...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Biochemical characterization and mechanistic...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Biochemical characterization and mechanistic...
questionsmedicales.fr › Phénomènes génétiques › Réparation de l'ADN › Biochemical characterization and mechanistic...
questionsmedicales.fr › Archéobactéries › Biochemical characterization and mechanistic...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Pyrimidines › Pyrimidinones › Uracile › Biochemical characterization and mechanistic...