Super-resolving microscopy reveals the localizations and movement dynamics of stressosome proteins in Listeria monocytogenes.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
14 01 2023
14 01 2023
Historique:
received:
12
10
2022
accepted:
04
01
2023
entrez:
14
1
2023
pubmed:
15
1
2023
medline:
18
1
2023
Statut:
epublish
Résumé
The human pathogen Listeria monocytogenes can cope with severe environmental challenges, for which the high molecular weight stressosome complex acts as the sensing hub in a complicated signal transduction pathway. Here, we show the dynamics and functional roles of the stressosome protein RsbR1 and its paralogue, the blue-light receptor RsbL, using photo-activated localization microscopy combined with single-particle tracking and single-molecule displacement mapping and supported by physiological studies. In live cells, RsbR1 is present in multiple states: in protomers with RsbS, large clusters of stressosome complexes, and in connection with the plasma membrane via Prli42. RsbL diffuses freely in the cytoplasm but forms clusters upon exposure to light. The clustering of RsbL is independent of the presence of Prli42. Our work provides a comprehensive view of the spatial organization and intracellular dynamics of the stressosome proteins in L. monocytogenes, which paves the way towards uncovering the stress-sensing mechanism of this signal transduction pathway.
Identifiants
pubmed: 36641529
doi: 10.1038/s42003-023-04423-y
pii: 10.1038/s42003-023-04423-y
pmc: PMC9840623
doi:
Substances chimiques
Bacterial Proteins
0
Phosphoproteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
51Informations de copyright
© 2023. The Author(s).
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