Glucocorticoids and medroxyprogesterone acetate synergize with inflammatory stimuli to selectively upregulate CCL20 transcription.


Journal

Molecular and cellular endocrinology
ISSN: 1872-8057
Titre abrégé: Mol Cell Endocrinol
Pays: Ireland
ID NLM: 7500844

Informations de publication

Date de publication:
01 03 2023
Historique:
received: 20 07 2022
revised: 29 11 2022
accepted: 12 01 2023
pmc-release: 01 03 2024
pubmed: 17 1 2023
medline: 2 2 2023
entrez: 16 1 2023
Statut: ppublish

Résumé

The pro-inflammatory cytokine, chemokine (C-C motif) ligand 20 (CCL20), is emerging as a therapeutic target for immune-based therapies. Cooperative regulation of CCL20 by glucocorticoids and progestins used in endocrine therapy and pro-inflammatory mediators could modulate immune function and affect disease outcomes. We show that glucocorticoids as well as medroxyprogesterone acetate (MPA), the progestin widely used in injectable contraception in sub-Saharan Africa, cooperate with pro-inflammatory mediators to upregulate CCL20 protein and/or mRNA in human peripheral blood mononuclear cells (PBMCs) and human cervical cell lines. Changes in CCL20 mRNA levels were shown to be synergistic, as assessed by Chou analysis, cell- and gene-specific and to involve transcriptional regulation, with a requirement for a nuclear factor kappa B (NF-κB) site and glucocorticoid receptor (GR) involvement. The novel results suggest a mechanism whereby MPA, like glucocorticoids, may impact inflammation both systemically and in the genital tract in patients using MPA and/or glucocorticoid therapy.

Identifiants

pubmed: 36646303
pii: S0303-7207(23)00006-0
doi: 10.1016/j.mce.2023.111855
pmc: PMC9892260
mid: NIHMS1867193
pii:
doi:

Substances chimiques

Medroxyprogesterone Acetate C2QI4IOI2G
Glucocorticoids 0
Progestins 0
Receptors, Glucocorticoid 0
RNA, Messenger 0
CCL20 protein, human 0
Chemokine CCL20 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

111855

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI152118
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD083026
Pays : United States

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no competing interests.

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Auteurs

Johnson M Moliki (JM)

Department of Molecular and Cell Biology, University of Cape Town, South Africa.

Tawanda J Nhundu (TJ)

Department of Molecular and Cell Biology, University of Cape Town, South Africa.

Leo Maritz (L)

Department of Molecular and Cell Biology, University of Cape Town, South Africa.

Chanel Avenant (C)

Department of Molecular and Cell Biology, University of Cape Town, South Africa.

Janet P Hapgood (JP)

Department of Molecular and Cell Biology, University of Cape Town, South Africa; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa. Electronic address: janet.hapgood@uct.ac.za.

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