Linear epitope mapping of the humoral response against SARS-CoV-2 in two independent African cohorts.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
16 01 2023
16 01 2023
Historique:
received:
25
07
2022
accepted:
09
01
2023
entrez:
16
1
2023
pubmed:
17
1
2023
medline:
19
1
2023
Statut:
epublish
Résumé
Profiling of the antibody responses to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) proteins in African populations is scarce. Here, we performed a detailed IgM and IgG epitope mapping study against 487 peptides covering SARS-CoV-2 wild-type structural proteins. A panel of 41 pre-pandemic and 82 COVID-19 RT-PCR confirmed sera from Madagascar and Senegal were used. We found that the main 36 immunodominant linear epitopes identified were (i) similar in both countries, (ii) distributed mainly in the Spike and the Nucleocapsid proteins, (iii) located outside the RBD and NTD regions where most of the reported SARS-CoV-2 variant mutations occur, and (iv) identical to those reported in European, North American, and Asian studies. Within the severe group, antibody levels were inversely correlated with the viral load. This first antibody epitope mapping study performed in patients from two African countries may be helpful to guide rational peptide-based diagnostic assays or vaccine development.
Identifiants
pubmed: 36646780
doi: 10.1038/s41598-023-27810-1
pii: 10.1038/s41598-023-27810-1
pmc: PMC9842613
doi:
Substances chimiques
Antibodies, Viral
0
Immunodominant Epitopes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
782Subventions
Organisme : Welcome Trust
ID : 221012/Z/20/Z
Informations de copyright
© 2023. The Author(s).
Références
Commun Biol. 2021 Feb 12;4(1):225
pubmed: 33580175
Science. 2020 Nov 27;370(6520):
pubmed: 32994364
ACS Cent Sci. 2020 Dec 23;6(12):2238-2249
pubmed: 33372199
PLoS One. 2020 Sep 9;15(9):e0238089
pubmed: 32903266
Eur J Immunol. 2021 Jul;51(7):1839-1849
pubmed: 33772767
Cell Rep. 2021 Mar 30;34(13):108915
pubmed: 33761319
Nature. 2020 Aug;584(7821):463-469
pubmed: 32717743
Nat Immunol. 2022 Feb;23(2):165-176
pubmed: 35105981
Bioinformatics. 2004 Aug 4;20 Suppl 1:i86-93
pubmed: 15262785
Nat Commun. 2020 Jun 1;11(1):2806
pubmed: 32483236
Science. 2020 Aug 7;369(6504):718-724
pubmed: 32661059
Am J Trop Med Hyg. 1994 Aug;51(2):123-37
pubmed: 8074247
Biomed Res Int. 2015;2015:904541
pubmed: 26125026
Nat Med. 2021 Jul;27(7):1280-1289
pubmed: 34017137
Sci Rep. 2021 Jul 28;11(1):15374
pubmed: 34321560
PLoS One. 2022 Sep 20;17(9):e0274783
pubmed: 36126041
Front Immunol. 2021 Mar 23;12:629185
pubmed: 33833755
Commun Biol. 2021 Nov 22;4(1):1317
pubmed: 34811480
Vaccines (Basel). 2021 Jan 11;9(1):
pubmed: 33440622
Emerg Microbes Infect. 2020 Dec;9(1):1988-1996
pubmed: 32844713
Influenza Other Respir Viruses. 2021 Jul;15(4):457-468
pubmed: 33586912
Influenza Other Respir Viruses. 2022 Jan;16(1):48-55
pubmed: 34378341
J Clin Med. 2021 Jun 30;10(13):
pubmed: 34209237
PLoS One. 2017 Jun 13;12(6):e0179146
pubmed: 28609450