Crystal structures of OrfX1, OrfX2 and the OrfX1-OrfX3 complex from the orfX gene cluster of botulinum neurotoxin E1.
OrfX1
OrfX2
OrfX3
bacterial toxin
botulinum neurotoxin
orfX gene cluster
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
02 2023
02 2023
Historique:
received:
07
12
2022
accepted:
12
12
2022
pmc-release:
01
02
2024
pubmed:
19
1
2023
medline:
3
3
2023
entrez:
18
1
2023
Statut:
ppublish
Résumé
Botulinum neurotoxins (BoNTs) are among the most lethal toxins known to humans, comprising seven established serotypes termed BoNT/A-G encoded in two types of gene clusters (ha and orfX) in BoNT-producing clostridia. The ha cluster encodes four non-toxic neurotoxin-associated proteins (NAPs) that assemble with BoNTs to protect and enhance their oral toxicity. However, the structure and function of the orfX-type NAPs remain largely unknown. Here, we report the crystal structures for OrfX1, OrfX2, and an OrfX1-OrfX3 complex, which are encoded in the orfX cluster of a BoNT/E1-producing Clostridium botulinum strain associated with human foodborne botulism. These structures lay the foundation for future studies on the potential roles of OrfX proteins in oral intoxication and pathogenesis of BoNTs.
Identifiants
pubmed: 36653893
doi: 10.1002/1873-3468.14576
pmc: PMC10019085
mid: NIHMS1877916
doi:
Substances chimiques
Botulinum Toxins, Type A
EC 3.4.24.69
Banques de données
RefSeq
['ACSC01000002']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
524-537Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM124165
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI091823
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI125704
Pays : United States
Organisme : NIH HHS
ID : S10 OD021527
Pays : United States
Informations de copyright
© 2023 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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