Chromodomain on Y-like 2 (CDYL2) implicated in mitosis and genome stability regulation via interaction with CHAMP1 and POGZ.
C13orf8
CAMP
Centromere
Epigenetics
ZNF828
Journal
Cellular and molecular life sciences : CMLS
ISSN: 1420-9071
Titre abrégé: Cell Mol Life Sci
Pays: Switzerland
ID NLM: 9705402
Informations de publication
Date de publication:
20 Jan 2023
20 Jan 2023
Historique:
received:
16
06
2022
accepted:
29
11
2022
revised:
28
11
2022
entrez:
19
1
2023
pubmed:
20
1
2023
medline:
24
1
2023
Statut:
epublish
Résumé
Histone H3 trimethylation on lysine 9 (H3K9me3) is a defining feature of mammalian pericentromeres, loss of which results in genome instability. Here we show that CDYL2 is recruited to pericentromeres in an H3K9me3-dependent manner and is required for faithful mitosis and genome stability. CDYL2 RNAi in MCF-7 breast cancer cells and Hela cervical cancer cells inhibited their growth, induced apoptosis, and provoked both nuclear and mitotic aberrations. Mass spectrometry analysis of CDYL2-interacting proteins identified the neurodevelopmental disease-linked mitotic regulators CHAMP1 and POGZ, which are associated with a central non-conserved region of CDYL2. RNAi rescue assays identified both the CDYL2 chromodomain and the CHAMP1-POGZ interacting region as required and, together, sufficient for CDYL2 regulation of mitosis and genome stability. CDYL2 RNAi caused loss of CHAMP1 localization at pericentromeres. We propose that CDYL2 functions as an adaptor protein that connects pericentromeric H3K9me3 with CHAMP1 and POGZ to ensure mitotic fidelity and genome stability.
Identifiants
pubmed: 36658409
doi: 10.1007/s00018-022-04659-7
pii: 10.1007/s00018-022-04659-7
doi:
Substances chimiques
CHAMP1 protein, human
0
Chromosomal Proteins, Non-Histone
0
Histones
0
Lysine
K3Z4F929H6
Phosphoproteins
0
CDYL2 protein, human
0
Co-Repressor Proteins
0
POGZ protein, human
0
Cell Cycle Proteins
0
DNA-Binding Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
47Subventions
Organisme : Institut National Du Cancer
ID : PLBIO2016-180
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
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