Fourier transform infrared spectroscopy; can it be used as a rapid typing method of Neisseria gonorrhoeae?

Humans Neisseria gonorrhoeae Multilocus Sequence Typing Spectroscopy, Fourier Transform Infrared Bacterial Typing Techniques / methods Gonorrhea / microbiology Microbial Sensitivity Tests Anti-Bacterial Agents / pharmacology Drug Resistance, Bacterial
Fourier transform infrared spectroscopy Neisseria gonorrhoeae molecular typing

Journal

Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883

Informations de publication

Date de publication:
02 2023
Historique:
received: 21 10 2022
revised: 16 12 2022
accepted: 15 01 2023
pubmed: 22 1 2023
medline: 9 2 2023
entrez: 21 1 2023
Statut: ppublish

Résumé

Typing of Neisseria gonorrhoeae is necessary for epidemiologic surveillance, while time consuming and resource intensive. Fourier transform infrared (FTIR) spectroscopy has shown promising results when typing several bacterial species. This study investigates whether FTIR spectroscopy can be used as a rapid method for typing clinical N. gonorrhoeae isolates, comparing FTIR spectroscopy to multi locus sequence typing (MLST), N. gonorrhoeae multi antigen sequence typing (NG-MAST) and whole genome sequencing (WGS). Sixty consecutive isolates from a venereology clinic and three isolates from an outbreak were included. Isolates were analysed with FTIR spectroscopy on the IR Biotyper system (Bruker Daltonik) with the IR Biotyper software (version 2.1) with default analysis settings (spectral range 1300-800 cm Fifty-one out of 60 isolates, and the three outbreak isolates, produced at least one spectrum in each run and were included. No agreement between FTIR spectroscopy and MLST or NG-MAST or WGS was shown. The FTIR spectroscopy-dendrogram failed to cluster the outbreak isolates. FTIR spectroscopy (spectral range 1300-800 cm

Sections du résumé

BACKGROUND
Typing of Neisseria gonorrhoeae is necessary for epidemiologic surveillance, while time consuming and resource intensive. Fourier transform infrared (FTIR) spectroscopy has shown promising results when typing several bacterial species. This study investigates whether FTIR spectroscopy can be used as a rapid method for typing clinical N. gonorrhoeae isolates, comparing FTIR spectroscopy to multi locus sequence typing (MLST), N. gonorrhoeae multi antigen sequence typing (NG-MAST) and whole genome sequencing (WGS).
METHODS
Sixty consecutive isolates from a venereology clinic and three isolates from an outbreak were included. Isolates were analysed with FTIR spectroscopy on the IR Biotyper system (Bruker Daltonik) with the IR Biotyper software (version 2.1) with default analysis settings (spectral range 1300-800 cm
RESULTS
Fifty-one out of 60 isolates, and the three outbreak isolates, produced at least one spectrum in each run and were included. No agreement between FTIR spectroscopy and MLST or NG-MAST or WGS was shown. The FTIR spectroscopy-dendrogram failed to cluster the outbreak isolates.
CONCLUSION
FTIR spectroscopy (spectral range 1300-800 cm

Identifiants

DOI: 10.1016/j.mimet.2023.106675 PMID: 36681126
pubmed: 36681126
pii: S0167-7012(23)00009-X
doi: 10.1016/j.mimet.2023.106675
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

106675

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest Norman Mauder is employed at Bruker Daltonik GmbH. The other authors have no relevant financial or non-financial interests to disclose.

Auteurs

Linn Merete Brendefur Corwin (LMB)

Microbiology Dept Rikshospitalet, Oslo University Hospital, PO Box 4950 Nydalen, 0424 Oslo, Norway; University of Oslo (UiO), Institute of Clinical Medicine, PO Box 1072 Blindern, 0316 Oslo, Norway. Electronic address: libren@ous-hf.no.

André Ingebretsen (A)

Microbiology Dept Rikshospitalet, Oslo University Hospital, PO Box 4950 Nydalen, 0424 Oslo, Norway. Electronic address: aingebre@ous-hf.no.

Patricia Campbell (P)

University of Oslo (UiO), Institute of Clinical Medicine, PO Box 1072 Blindern, 0316 Oslo, Norway; Microbiology Dept, Akershus University Hospital, PO Box 1000, 1478 Lørenskog, Norway. Electronic address: Patricia.Campbell@ahus.no.

Kristian Alfsnes (K)

Norwegian Institute of Public Health (NIPH), PO Box 222 Skøyen, 0213 Oslo, Norway. Electronic address: Kristian.Alfsnes@fhi.no.

Fredrik Müller (F)

Microbiology Dept Rikshospitalet, Oslo University Hospital, PO Box 4950 Nydalen, 0424 Oslo, Norway; University of Oslo (UiO), Institute of Clinical Medicine, PO Box 1072 Blindern, 0316 Oslo, Norway. Electronic address: fmuller@ous-hf.no.

Norman Mauder (N)

Bruker Daltonics GmbH & Co. KG, Fahrenheitstrasse 4, 28359 Bremen, Germany. Electronic address: Norman.Mauder@bruker.com.

Michael Koomey (M)

University of Oslo (UiO), Department of Biosciences, PO Box 1072 Blindern, 0316 Oslo, Norway. Electronic address: j.m.koomey@ibv.uio.no.

Jørgen Vildershøj Bjørnholt (JV)

Microbiology Dept Rikshospitalet, Oslo University Hospital, PO Box 4950 Nydalen, 0424 Oslo, Norway; University of Oslo (UiO), Institute of Clinical Medicine, PO Box 1072 Blindern, 0316 Oslo, Norway. Electronic address: joerbj@ous-hf.no.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Fourier transform infrared spectroscopy; can...
questionsmedicales.fr › Bactéries › Proteobacteria › Betaproteobacteria › Neisseriaceae › Neisseria › Neisseria gonorrhoeae › Fourier transform infrared spectroscopy; can...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Typage par séquençage multilocus › Fourier transform infrared spectroscopy; can...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrophotométrie › Spectrophotométrie IR › Spectroscopie infrarouge à transformée de Fourier › Fourier transform infrared spectroscopy; can...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques microbiologiques › Techniques bactériologiques › Techniques de typage bactérien › Fourier transform infrared spectroscopy; can...
questionsmedicales.fr › Maladies urogénitales › Maladies génitales › Gonorrhée › Fourier transform infrared spectroscopy; can...
questionsmedicales.fr › Techniques d'investigation › Évaluation préclinique de médicament › Tests de sensibilité microbienne › Fourier transform infrared spectroscopy; can...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Fourier transform infrared spectroscopy; can...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Résistance microbienne aux médicaments › Résistance bactérienne aux médicaments › Fourier transform infrared spectroscopy; can...