Analysis of regulatory sequences in exosomal DNA of NANOGP8.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2023
2023
Historique:
received:
15
03
2022
accepted:
20
12
2022
entrez:
25
1
2023
pubmed:
26
1
2023
medline:
28
1
2023
Statut:
epublish
Résumé
Exosomes participate in intercellular communication by transporting functionally active molecules. Such cargo from the original cells comprising proteins, micro-RNA, mRNA, single-stranded (ssDNA) and double-stranded DNA (dsDNA) molecules pleiotropically transforms the target cells. Although cancer cells secrete exosomes carrying a significant level of DNA capable of modulating oncogene expression in a recipient cell, the regulatory mechanism is unknown. We have previously reported that cancer cells produce exosomes containing NANOGP8 DNA. NANOGP8 is an oncogenic paralog of embryonic stem cell transcription factor NANOG and does not express in cells since it is a pseudogene. However, in this study, we evaluated NANOGP8 expression in glioblastoma multiforme (GBM) tissue from a surgically removed brain tumor of a patient. Significantly higher NANOGP8 transcription was observed in GBM cancer stem cells (CSCs) than in GBM cancer cells or neural stem cells (NSCs), despite identical sequences of NANOGP8-upstream genomic region in all the cell lines. This finding suggests that upstream genomic sequences of NANOGP8 may have environment-dependent promoter activity. We also found that the regulatory sequences upstream of exosomal NANOGP8 GBM DNA contain multiple core promoter elements, transcription factor binding sites, and segments of human viruses known for their oncogenic role. The exosomal sequence of NANOGP8-upstream GBM DNA is different from corresponding genomic sequences in CSCs, cancer cells, and NSCs as well as from the sequences reported by NCBI. These sequence dissimilarities suggest that exosomal NANOGP8 GBM DNA may not be a part of the genomic DNA. Exosomes possibly acquire this DNA from other sources where it is synthesized by an unknown mechanism. The significance of exosome-bestowed regulatory elements in the transcription of promoter-less retrogene such as NANOGP8 remains to be determined.
Identifiants
pubmed: 36696426
doi: 10.1371/journal.pone.0280959
pii: PONE-D-22-07639
pmc: PMC9876286
doi:
Substances chimiques
Nanog Homeobox Protein
0
MicroRNAs
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0280959Informations de copyright
Copyright: © 2023 Vaidya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Cell Stem Cell. 2012 Dec 7;11(6):836-44
pubmed: 23217424
ACS Nano. 2019 Dec 24;13(12):13853-13865
pubmed: 31479239
EMBO J. 2010 Aug 4;29(15):2659-74
pubmed: 20581802
Viruses. 2010 Jan;2(1):111-130
pubmed: 21994603
Cancers (Basel). 2021 Jul 29;13(15):
pubmed: 34359729
Transcription. 2015;6(3):41-50
pubmed: 26226151
Cell. 1988 Dec 2;55(5):917-24
pubmed: 3142692
Biomedicines. 2020 Jul 16;8(7):
pubmed: 32708613
Stem Cells. 2012 Oct;30(10):2076-87
pubmed: 22851508
Steroids. 2010 Jan;75(1):1-12
pubmed: 19818358
J Exp Clin Cancer Res. 2020 Sep 11;39(1):184
pubmed: 32917236
Int J Mol Sci. 2018 Dec 04;19(12):
pubmed: 30518090
Stem Cells. 2015 Jun;33(6):1705-18
pubmed: 25754111
Bioinformatics. 2008 Jul 15;24(14):1563-7
pubmed: 18535086
BMC Biol. 2016 Jun 13;14:46
pubmed: 27296830
J Biol Chem. 1998 Jan 9;273(2):1086-91
pubmed: 9422773
Infect Agent Cancer. 2015 Jan 27;10:3
pubmed: 25685179
Iran J Basic Med Sci. 2016 Oct;19(10):1031-1039
pubmed: 27872698
Methods Mol Biol. 2021;2324:255-262
pubmed: 34165720
Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):10827-31
pubmed: 22711823
J Biol Chem. 1997 Oct 17;272(42):26188-95
pubmed: 9334186
Sci Rep. 2016 Apr 12;6:23978
pubmed: 27068479
PLoS Biol. 2019 Mar 18;17(3):e3000197
pubmed: 30883542
PLoS One. 2013 Aug 06;8(8):e68712
pubmed: 23936309
Cancer Drug Resist. 2021 Mar 19;4(1):1-16
pubmed: 35582008
J Virol. 1998 Aug;72(8):6592-601
pubmed: 9658104
Genes Dev. 2010 Jun 1;24(11):1106-18
pubmed: 20516196
Mol Cancer. 2003 Aug 20;2:29
pubmed: 12971829
PLoS One. 2018 May 22;13(5):e0197782
pubmed: 29787607
Am J Cancer Res. 2020 Oct 01;10(10):3083-3105
pubmed: 33163259
Biochimie. 2020 Jan;168:1-9
pubmed: 31626853
Cell Death Dis. 2016 Apr 21;7:e2199
pubmed: 27100893
Front Med (Lausanne). 2015 Sep 25;2:68
pubmed: 26442270
Int J Biol Sci. 2015 Aug 01;11(10):1160-70
pubmed: 26327810
Adv Exp Med Biol. 2020;1202:203-222
pubmed: 32034715
Nucleic Acids Res. 2003 Jul 1;31(13):3651-3
pubmed: 12824386
Viruses. 2017 Apr 13;9(4):
pubmed: 28406439
Oncogene. 2014 May 8;33(19):2513-9
pubmed: 23752184
Gene Expr. 1999;8(2):67-84
pubmed: 10551796
Bioinformatics. 2002 Feb;18(2):333-4
pubmed: 11847087
Int J Biol Sci. 2021 Jan 1;17(1):163-177
pubmed: 33390841
Adv Biol Regul. 2014 Sep;56:30-44
pubmed: 24931719
Bioinformatics. 2005 Jul 1;21(13):2933-42
pubmed: 15860560
Int J Dev Biol. 2010;54(11-12):1743-54
pubmed: 21136380
Oncogene. 2013 Sep 12;32(37):4397-405
pubmed: 23085761
Hum Mol Genet. 2011 Nov 15;20(22):4299-310
pubmed: 21835883
Clin Neurosurg. 2010;57:151-9
pubmed: 21280509
Curr Protoc Cell Biol. 2006 Apr;Chapter 3:Unit 3.22
pubmed: 18228490
IUBMB Life. 2006 Dec;58(12):677-85
pubmed: 17424906
Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20563-8
pubmed: 24297917
Oncol Lett. 2016 Oct;12(4):2277-2282
pubmed: 27698789
J Virol. 1993 Apr;67(4):1967-75
pubmed: 8445718
Stem Cells. 2013 May;31(5):857-69
pubmed: 23307586
G3 (Bethesda). 2012 Nov;2(11):1447-57
pubmed: 23173096
Cureus. 2018 Oct 11;10(10):e3439
pubmed: 30555755
J Virol. 2006 Apr;80(8):4026-37
pubmed: 16571819
Life (Basel). 2021 Jan 19;11(1):
pubmed: 33478113
Philos Trans R Soc Lond B Biol Sci. 2020 Sep 14;375(1807):20190390
pubmed: 32713306
Cancers (Basel). 2021 Jan 15;13(2):
pubmed: 33467638
Theranostics. 2020 Jan 1;10(4):1479-1499
pubmed: 32042317
Oncogene. 2012 Nov 1;31(44):4655-66
pubmed: 22249269
Mol Biol (Mosk). 2008 May-Jun;42(3):388-404
pubmed: 18702298
Cells. 2016 Apr 06;5(2):
pubmed: 27058560
Nat Rev Genet. 2019 Apr;20(4):207-220
pubmed: 30675018
FEBS J. 2006 Apr;273(8):1723-30
pubmed: 16623708
Nat Commun. 2020 Apr 29;11(1):2092
pubmed: 32350252
Cancers (Basel). 2017 Jan 12;9(1):
pubmed: 28085080
PLoS One. 2018 Apr 24;13(4):e0192436
pubmed: 29689047
Cell. 2017 Feb 9;168(4):629-643
pubmed: 28187285
Genome Biol. 2011 Dec 28;12(12):236
pubmed: 22204421
Cancer Gene Ther. 2009 Feb;16(2):103-12
pubmed: 18846113
PLoS One. 2014 Jul 09;9(7):e102117
pubmed: 25007265
In Vivo. 2017 Mar-Apr;31(2):221-229
pubmed: 28358704
Retrovirology. 2013 Nov 12;10:132
pubmed: 24219971
Sci Adv. 2020 Nov 20;6(47):
pubmed: 33219024
Fluids Barriers CNS. 2022 Jul 25;19(1):60
pubmed: 35879759
Cell Host Microbe. 2014 Mar 12;15(3):266-82
pubmed: 24629334
Int J Mol Sci. 2021 Jun 15;22(12):
pubmed: 34203727
BMC Res Notes. 2014 Mar 19;7:159
pubmed: 24642114
Front Cell Dev Biol. 2021 Aug 16;9:695325
pubmed: 34485282
Neuro Oncol. 2014 Jan;16(2):298-302
pubmed: 24285549
Cell Mol Life Sci. 2015 Jan;72(1):1-10
pubmed: 25156068
Cell. 2009 Aug 21;138(4):722-37
pubmed: 19703398
Genes Dev. 2018 Jan 1;32(1):1-3
pubmed: 29440223
BMC Bioinformatics. 2006 Mar 07;7:114
pubmed: 16522199
Front Genet. 2019 Oct 01;10:906
pubmed: 31632439
Front Immunol. 2020 Jan 21;10:3137
pubmed: 32038644
Curr Mol Med. 2016;16(4):353-93
pubmed: 26980699
Blood Cells Mol Dis. 2004 Jan-Feb;32(1):143-54
pubmed: 14757430
Cell Res. 2007 Jan;17(1):42-9
pubmed: 17211451
J Virol. 2009 Jun;83(12):6098-105
pubmed: 19339349
Oncotarget. 2015 Oct 13;6(31):31479-92
pubmed: 26362401
J Virol. 2008 Oct;82(20):9964-77
pubmed: 18684813
Am J Physiol Gastrointest Liver Physiol. 2005 Feb;288(2):G175-81
pubmed: 15458926
Nat Rev Genet. 2009 Jan;10(1):19-31
pubmed: 19030023
Neuro Oncol. 2021 Oct 5;23(12 Suppl 2):iii1-iii105
pubmed: 34608945