One-step impedimetric NT-proBNP aptasensor targeting cardiac insufficiency in artificial saliva.
Artificial saliva
Cardiac insufficiency
Electrochemical impedance spectroscopy
Label-free aptasensor
N-terminal pro b-type natriuretic peptide
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
01 May 2023
01 May 2023
Historique:
received:
15
11
2022
revised:
16
01
2023
accepted:
17
01
2023
pubmed:
26
1
2023
medline:
22
2
2023
entrez:
25
1
2023
Statut:
ppublish
Résumé
Currently, sensitive and accurate approaches for diagnosis, rapid assessment, and cardiac biomarker monitoring in patients with heart failure are needed. In this context, the advantages of aptamers over traditional antibodies have been employed to fabricate a single-step impedimetric N-terminal pro b-type natriuretic peptide (NT-proBNP)-modified gold microelectrode array. The development of an electrochemical aptasensing platform was based on the coimmobilization of alkanethiol self-assembled monolayers and amine-terminated aptamer that specifically recognized cardiac NT-proBNP protein resulting in charge electron transfer. Electroimpedimetric signals of the sensor were observed to be linear to the NT-proBNP concentrations in the range of 5.0 × 10
Identifiants
pubmed: 36696735
pii: S0039-9140(23)00031-0
doi: 10.1016/j.talanta.2023.124280
pii:
doi:
Substances chimiques
pro-brain natriuretic peptide (1-76)
0
Natriuretic Peptide, Brain
114471-18-0
Saliva, Artificial
0
Peptide Fragments
0
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
124280Informations de copyright
Copyright © 2023. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.