Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
27 01 2023
27 01 2023
Historique:
received:
20
05
2022
accepted:
10
01
2023
pubmed:
28
1
2023
medline:
1
2
2023
entrez:
27
1
2023
Statut:
epublish
Résumé
The steep increase in nontuberculous mycobacteria (NTM) infections makes understanding their unique physiology an urgent health priority. NTM synthesize two polysaccharides proposed to modulate fatty acid metabolism: the ubiquitous 6-O-methylglucose lipopolysaccharide, and the 3-O-methylmannose polysaccharide (MMP) so far detected in rapidly growing mycobacteria. The recent identification of a unique MMP methyltransferase implicated the adjacent genes in MMP biosynthesis. We report a wide distribution of this gene cluster in NTM, including slowly growing mycobacteria such as Mycobacterium avium, which we reveal to produce MMP. Using a combination of MMP purification and chemoenzymatic syntheses of intermediates, we identified the biosynthetic mechanism of MMP, relying on two enzymes that we characterized biochemically and structurally: a previously undescribed α-endomannosidase that hydrolyses MMP into defined-sized mannoligosaccharides that prime the elongation of new daughter MMP chains by a rare α-(1→4)-mannosyltransferase. Therefore, MMP biogenesis occurs through a partially conservative replication mechanism, whose disruption affected mycobacterial growth rate at low temperature.
Identifiants
pubmed: 36707645
doi: 10.1038/s42003-023-04448-3
pii: 10.1038/s42003-023-04448-3
pmc: PMC9883506
doi:
Substances chimiques
methylmannoside
51023-63-3
Lipopolysaccharides
0
Mannosyltransferases
EC 2.4.1.-
Methyltransferases
EC 2.1.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108Informations de copyright
© 2023. The Author(s).
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