Multiple opsins in a reef-building coral, Acropora millepora.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 01 2023
29 01 2023
Historique:
received:
27
05
2022
accepted:
19
01
2023
entrez:
29
1
2023
pubmed:
30
1
2023
medline:
1
2
2023
Statut:
epublish
Résumé
Opsins, light-sensitive G protein-coupled receptors, have been identified in corals but their properties are largely unknown. Here, we identified six opsin genes (acropsins 1-6) from a coral species Acropora millepora, including three novel opsins (acropsins 4-6), and successfully characterized the properties of four out of the six acropsins. Acropsins 1 and 6 exhibited light-dependent cAMP increases in cultured cells, suggesting that the acropsins could light-dependently activate Gs-type G protein like the box jellyfish opsin from the same opsin group. Spectral sensitivity curves having the maximum sensitivities at ~ 472 nm and ~ 476 nm were estimated for acropsins 1 and 6, respectively, based on the light wavelength-dependent cAMP increases in these opsins-expressing cells (heterologous action spectroscopy). Acropsin 2 belonging to the same group as acropsins 1 and 6 did not induce light-dependent cAMP or Ca
Identifiants
pubmed: 36710295
doi: 10.1038/s41598-023-28476-5
pii: 10.1038/s41598-023-28476-5
pmc: PMC9884665
doi:
Substances chimiques
Opsins
0
Rod Opsins
0
GTP-Binding Proteins
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1628Informations de copyright
© 2023. The Author(s).
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