Grain shattering by cell death and fracture in Eragrostis tef.
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
02 05 2023
02 05 2023
Historique:
received:
18
10
2022
accepted:
11
01
2023
medline:
4
5
2023
pubmed:
10
2
2023
entrez:
9
2
2023
Statut:
ppublish
Résumé
Abscission, known as shattering in crop species, is a highly regulated process by which plants shed parts. Although shattering has been studied extensively in cereals and a number of regulatory genes have been identified, much diversity in the process remains to be discovered. Teff (Eragrostis tef) is a crop native to Ethiopia that is potentially highly valuable worldwide for its nutritious grain and drought tolerance. Previous work has suggested that grain shattering in Eragrostis might have little in common with other cereals. In this study, we characterize the anatomy, cellular structure, and gene regulatory control of the abscission zone (AZ) in E. tef. We show that the AZ of E. tef is a narrow stalk below the caryopsis, which is common in Eragrostis species. X-ray microscopy, scanning electron microscopy, transmission electron microscopy, and immunolocalization of cell wall components showed that the AZ cells are thin walled and break open along with programmed cell death (PCD) at seed maturity, rather than separating between cells as in other studied species. Knockout of YABBY2/SHATTERING1, documented to control abscission in several cereals, had no effect on abscission or AZ structure in E. tef. RNA sequencing analysis showed that genes related to PCD and cell wall modification are enriched in the AZ at the early seed maturity stage. These data show that E. tef drops its seeds using a unique mechanism. Our results provide the groundwork for understanding grain shattering in Eragrostis and further improvement of shattering in E. tef.
Identifiants
pubmed: 36756804
pii: 7032508
doi: 10.1093/plphys/kiad079
pmc: PMC10152664
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
222-239Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.
Déclaration de conflit d'intérêts
Conflict of interest statement. The authors declare no conflict of interest.
Références
J Exp Bot. 2007;58(13):3719-30
pubmed: 17928369
Curr Top Dev Biol. 2016;119:63-109
pubmed: 27282024
Front Plant Sci. 2015 Jun 24;6:476
pubmed: 26157453
Am J Bot. 2016 Jun;103(6):998-1005
pubmed: 27257006
Plant Physiol. 2022 Feb 4;188(2):831-845
pubmed: 34618094
Genome Biol. 2015 Aug 06;16:157
pubmed: 26243257
Cell. 2018 May 31;173(6):1468-1480.e9
pubmed: 29731167
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Plant Physiol. 1997 Apr;113(4):1303-8
pubmed: 9112778
Nat Biotechnol. 2020 Oct;38(10):1203-1210
pubmed: 33020633
Plant Physiol. 1996 Jul;111(3):813-20
pubmed: 8754682
Cell. 2015 Jul 30;162(3):527-39
pubmed: 26232223
Planta. 2007 Oct;226(5):1195-205
pubmed: 17618454
Nat Genet. 2012 May 13;44(6):720-4
pubmed: 22581231
Plant Physiol Biochem. 2008 Jan;46(1):54-63
pubmed: 17964177
Plant Physiol. 2001 Jun;126(2):494-500
pubmed: 11402180
Plant Cell. 2011 Nov;23(11):4146-63
pubmed: 22128123
Science. 2006 Mar 31;311(5769):1936-9
pubmed: 16527928
Plant Signal Behav. 2014;9(11):e976154
pubmed: 25482787
Development. 2008 Apr;135(8):1537-46
pubmed: 18339677
Science. 2006 Jun 2;312(5778):1392-6
pubmed: 16614172
Plant Physiol. 1997 Aug;114(4):1541-6
pubmed: 9276961
Plant Physiol. 2006 Oct;142(2):710-21
pubmed: 16920876
Mol Biol Evol. 2022 Jun 2;39(6):
pubmed: 35388422
Front Genet. 2021 Dec 13;12:784545
pubmed: 34966414
Front Plant Sci. 2015 Jul 14;6:523
pubmed: 26236321
Nat Commun. 2020 Feb 14;11(1):884
pubmed: 32060277
Genome Biol. 2006;7(9):R87
pubmed: 17010199
OMICS. 2012 May;16(5):284-7
pubmed: 22455463
Genome Biol. 2014;15(12):550
pubmed: 25516281
EMBO Rep. 2005 Mar;6(3):282-8
pubmed: 15723040
Plant Cell Rep. 2017 Sep;36(9):1477-1491
pubmed: 28681159
Front Plant Sci. 2014 Jul 28;5:366
pubmed: 25120551
Plant Biotechnol J. 2022 Sep;20(9):1716-1729
pubmed: 35560779
Plant Physiol. 2012 Jun;159(2):835-50
pubmed: 22492844
Front Plant Sci. 2017 Feb 08;8:126
pubmed: 28228766
Front Plant Sci. 2018 Jul 19;9:918
pubmed: 30073004
Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9783-8
pubmed: 10931949
Planta. 2019 Sep;250(3):753-767
pubmed: 31222492
J Exp Bot. 2011 Oct;62(14):5091-103
pubmed: 21765161
Nat Plants. 2018 Jun;4(6):331-337
pubmed: 29872176
Plant Cell. 2017 Jun;29(6):1196-1217
pubmed: 28522548
Plant Physiol. 2009 Feb;149(2):775-90
pubmed: 19098090
Nucleic Acids Res. 2018 Jul 2;46(W1):W242-W245
pubmed: 29762716
Plant Cell. 2016 Sep;28(9):1998-2015
pubmed: 27600536
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
Front Plant Sci. 2017 Apr 19;8:606
pubmed: 28469634
Am J Bot. 2020 Apr;107(4):549-561
pubmed: 32207156
J Exp Bot. 2019 Oct 15;70(19):5189-5203
pubmed: 31173099
New Phytol. 2020 Feb;225(4):1799-1815
pubmed: 31372996
Proc Natl Acad Sci U S A. 2014 Apr 29;111(17):6178-83
pubmed: 24753598
J Histochem Cytochem. 2005 Apr;53(4):543-6
pubmed: 15805428
BMC Plant Biol. 2012 Aug 25;12:150
pubmed: 22920238
Annu Rev Cell Dev Biol. 2016 Oct 6;32:441-468
pubmed: 27298090
Plant Cell. 2009 Jan;21(1):216-33
pubmed: 19168715
Plant Mol Biol. 1995 Jul;28(4):647-56
pubmed: 7647297
Plant J. 2014 Sep;79(5):717-28
pubmed: 24923192
Front Plant Sci. 2016 Apr 26;7:540
pubmed: 27200017
Sci Rep. 2020 Sep 10;10(1):14936
pubmed: 32913300
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
J Exp Bot. 2011 Mar;62(5):1633-49
pubmed: 21325605
Cell Death Differ. 2011 Aug;18(8):1241-6
pubmed: 21494263
Plant Physiol. 1988 Dec;88(4):1257-62
pubmed: 16666452
Plant Methods. 2021 May 25;17(1):54
pubmed: 34034755
J Exp Bot. 2008;59(4):973-9
pubmed: 18316316
J Exp Bot. 2009;60(7):2035-44
pubmed: 19346241
BMC Bioinformatics. 2008 Dec 29;9:559
pubmed: 19114008
Curr Opin Plant Biol. 2000 Feb;3(1):17-22
pubmed: 10679447
Plant Physiol. 2002 Feb;128(2):534-43
pubmed: 11842157
Plant Cell. 2012 Mar;24(3):1034-48
pubmed: 22408071
Plant Mol Biol. 1999 Jul;40(4):615-22
pubmed: 10480385
Am J Bot. 2003 Jan;90(1):116-22
pubmed: 21659086
Annu Rev Plant Biol. 2002;53:131-58
pubmed: 12221970