Community RNA-Seq: multi-kingdom responses to living versus decaying roots in soil.


Journal

ISME communications
ISSN: 2730-6151
Titre abrégé: ISME Commun
Pays: England
ID NLM: 9918205372406676

Informations de publication

Date de publication:
06 Dec 2021
Historique:
received: 19 02 2021
accepted: 23 09 2021
revised: 14 09 2021
entrez: 10 2 2023
pubmed: 6 12 2021
medline: 6 12 2021
Statut: epublish

Résumé

Roots are a primary source of organic carbon input in most soils. The consumption of living and detrital root inputs involves multi-trophic processes and multiple kingdoms of microbial life, but typical microbial ecology studies focus on only one or two major lineages. We used Illumina shotgun RNA sequencing to conduct PCR-independent SSU rRNA community analysis ("community RNA-Seq") and simultaneously assess the bacteria, archaea, fungi, and microfauna surrounding both living and decomposing roots of the annual grass, Avena fatua. Plants were grown in

Identifiants

pubmed: 36765158
doi: 10.1038/s43705-021-00059-3
pii: 10.1038/s43705-021-00059-3
pmc: PMC9723751
doi:

Types de publication

Journal Article

Langues

eng

Pagination

72

Subventions

Organisme : DOE | SC | Biological and Environmental Research (BER)
ID : SCW1711
Organisme : DOE | SC | Biological and Environmental Research (BER)
ID : DE-SC0010570
Organisme : DOE | SC | Biological and Environmental Research (BER)
ID : DE-SC0016247
Organisme : DOE | SC | Biological and Environmental Research (BER)
ID : DE-SC0020163
Organisme : DOE | SC | Biological and Environmental Research (BER)
ID : SCW1421
Organisme : DOE | SC | Biological and Environmental Research (BER)
ID : SCW1589
Organisme : DOE | SC | Biological and Environmental Research (BER)
ID : SCW1678

Informations de copyright

© 2021. © Lawrence Livermore National Laboratory 2021.

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Auteurs

Erin E Nuccio (EE)

Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA. nuccio1@llnl.gov.

Nhu H Nguyen (NH)

Department of Tropical Plant and Soil Sciences, University of Hawai'i at Mānoa, Honolulu, HI, USA.

Ulisses Nunes da Rocha (U)

Department of Environmental Microbiology, Helmholtz Centre for Environmental Research, Leipzig, Germany.

Xavier Mayali (X)

Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.

Jeremy Bougoure (J)

Centre for Microscopy, Characterisation & Analysis, The University of Western Australia, Perth, Australia.

Peter K Weber (PK)

Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.

Eoin Brodie (E)

Earth and Environmental Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, USA.

Mary Firestone (M)

Earth and Environmental Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, USA.

Jennifer Pett-Ridge (J)

Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA. pettridge2@llnl.gov.
Life and Environmental Sciences Department, University of California Merced, Merced, CA, USA. pettridge2@llnl.gov.

Classifications MeSH