Principles of target DNA cleavage and the role of Mg2+ in the catalysis of CRISPR-Cas9.
Journal
Nature catalysis
ISSN: 2520-1158
Titre abrégé: Nat Catal
Pays: England
ID NLM: 101714581
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
entrez:
13
2
2023
pubmed:
14
2
2023
medline:
14
2
2023
Statut:
ppublish
Résumé
At the core of the CRISPR-Cas9 genome-editing technology, the endonuclease Cas9 introduces site-specific breaks in DNA. However, precise mechanistic information to ameliorating Cas9 function is still missing. Here, multi-microsecond molecular dynamics, free-energy and multiscale simulations are combined with solution NMR and DNA cleavage experiments to resolve the catalytic mechanism of target DNA cleavage. We show that the conformation of an active HNH nuclease is tightly dependent on the catalytic Mg
Identifiants
pubmed: 36778082
doi: 10.1038/s41929-022-00848-6
pmc: PMC9909973
mid: NIHMS1852788
doi:
Types de publication
Journal Article
Langues
eng
Pagination
912-922Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM136815
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM141329
Pays : United States
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