Nitrogenase resurrection and the evolution of a singular enzymatic mechanism.
Azotobacter vinelandii
ancestral sequence reconstruction
azotobacter vinelandii
biochemistry
chemical biology
early life
evolution
evolutionary biochemistry
evolutionary biology
metabolic engineering
nitrogen fixation
nitrogenase
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
17 02 2023
17 02 2023
Historique:
received:
18
11
2022
accepted:
16
02
2023
pubmed:
18
2
2023
medline:
4
3
2023
entrez:
17
2
2023
Statut:
epublish
Résumé
The planetary biosphere is powered by a suite of key metabolic innovations that emerged early in the history of life. However, it is unknown whether life has always followed the same set of strategies for performing these critical tasks. Today, microbes access atmospheric sources of bioessential nitrogen through the activities of just one family of enzymes, nitrogenases. Here, we show that the only dinitrogen reduction mechanism known to date is an ancient feature conserved from nitrogenase ancestors. We designed a paleomolecular engineering approach wherein ancestral nitrogenase genes were phylogenetically reconstructed and inserted into the genome of the diazotrophic bacterial model,
Identifiants
pubmed: 36799917
doi: 10.7554/eLife.85003
pii: 85003
pmc: PMC9977276
doi:
pii:
Substances chimiques
Nitrogenase
EC 1.18.6.1
Nitrogen
N762921K75
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NASA
ID : Postdoctoral Fellowship
Pays : United States
Organisme : NASA
ID : 19- ICAR19_2-0007
Pays : United States
Organisme : NASA
ID : 80NSSC19K1617
Pays : United States
Informations de copyright
© 2023, Garcia et al.
Déclaration de conflit d'intérêts
AG, DH, AR, BC, AP, LS, BK No competing interests declared
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