Biosorption of process-equipment-related leachables (PERLs) in biomanufacturing: A quantitative approach to study partitioning of PERLs in a cell culture system.

CAR-T Manufacturing Cell Culture Extractables and Leachables Partitioning Single-Use Systems

Journal

International journal of pharmaceutics
ISSN: 1873-3476
Titre abrégé: Int J Pharm
Pays: Netherlands
ID NLM: 7804127

Informations de publication

Date de publication:
25 Mar 2023
Historique:
received: 11 11 2022
revised: 11 02 2023
accepted: 14 02 2023
pubmed: 23 2 2023
medline: 21 3 2023
entrez: 22 2 2023
Statut: ppublish

Résumé

The assessment and potential risk of process equipment-related leachables (PERLs) in the production of biopharmaceuticals and cell therapeutics using single-use (SU) equipment has been discussed previously. However, potential interactions of cells with PERLs have not yet been considered. Here, we present a quantitative adsorption study of neutral, organic small-molecule leachable compounds - known for extractables & leachables (E&L) analysis of SU equipment - in aqueous suspensions of CHO and T cells. The solid-water partition coefficient K

Identifiants

pubmed: 36804518
pii: S0378-5173(23)00162-X
doi: 10.1016/j.ijpharm.2023.122742
pii:
doi:

Substances chimiques

Organic Chemicals 0
Water 059QF0KO0R

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

122742

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

M Bossong (M)

Department of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Mainz, Germany; Sartorius Stedim Biotech GmbH, August-Spindler-Straße 11, 37079 Göttingen, Germany. Electronic address: mbossong@uni-mainz.de.

D Budde (D)

Sartorius Stedim Biotech GmbH, August-Spindler-Straße 11, 37079 Göttingen, Germany.

A Hauk (A)

Sartorius Stedim Biotech GmbH, August-Spindler-Straße 11, 37079 Göttingen, Germany.

I Pahl (I)

Sartorius Stedim Biotech GmbH, August-Spindler-Straße 11, 37079 Göttingen, Germany.

R Menzel (R)

Sartorius Stedim Biotech GmbH, August-Spindler-Straße 11, 37079 Göttingen, Germany.

P Langguth (P)

Department of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Mainz, Germany.

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Classifications MeSH