High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq.
Journal
Nature biotechnology
ISSN: 1546-1696
Titre abrégé: Nat Biotechnol
Pays: United States
ID NLM: 9604648
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
28
03
2022
accepted:
12
01
2023
pubmed:
25
2
2023
medline:
25
2
2023
entrez:
24
2
2023
Statut:
ppublish
Résumé
In this study, we extended co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension and demonstrated high-plex protein and whole transcriptome co-mapping. We profiled 189 proteins and whole transcriptome in multiple mouse tissue types with spatial CITE sequencing and then further applied the method to measure 273 proteins and transcriptome in human tissues, revealing spatially distinct germinal center reactions in tonsil and early immune activation in skin at the Coronavirus Disease 2019 mRNA vaccine injection site.
Identifiants
pubmed: 36823353
doi: 10.1038/s41587-023-01676-0
pii: 10.1038/s41587-023-01676-0
pmc: PMC10567548
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1405-1409Subventions
Organisme : NIMH NIH HHS
ID : RF1 MH128876
Pays : United States
Organisme : NCI NIH HHS
ID : UG3 CA257393
Pays : United States
Organisme : NCI NIH HHS
ID : UH3 CA257393
Pays : United States
Organisme : NIA NIH HHS
ID : U54 AG076043
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA245313
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023. The Author(s).
Références
Science. 2016 Jul 1;353(6294):78-82
pubmed: 27365449
Elife. 2018 Jul 11;7:
pubmed: 29993362
Sci Rep. 2019 Mar 14;9(1):4489
pubmed: 30872751
Cell. 2018 Aug 9;174(4):968-981.e15
pubmed: 30078711
Nat Rev Immunol. 2002 Aug;2(8):580-92
pubmed: 12154377
Nat Biotechnol. 2023 Jun;41(6):788-793
pubmed: 36593397
Nat Commun. 2022 Feb 10;13(1):795
pubmed: 35145087
Cell. 2022 Jan 20;185(2):379-396.e38
pubmed: 35021063
Front Immunol. 2021 Dec 02;12:744573
pubmed: 34925321
Immunity. 2016 May 17;44(5):989-1004
pubmed: 27192565
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Cell Mol Immunol. 2021 Mar;18(3):523-527
pubmed: 32868910
Nat Cancer. 2022 Jan;3(1):122-133
pubmed: 35121992
Curr Protoc Chem Biol. 2016 Dec 7;8(4):251-264
pubmed: 27925668
Cell. 2020 Dec 10;183(6):1665-1681.e18
pubmed: 33188776
Nucleic Acids Res. 2021 May 21;49(9):e50
pubmed: 33544846
Semin Immunol. 2008 Feb;20(1):43-8
pubmed: 18243730
STAR Protoc. 2021 May 11;2(2):100532
pubmed: 34027489
Microsyst Nanoeng. 2019 Nov 6;5:59
pubmed: 31700674
Bioinformatics. 2017 Aug 15;33(16):2591-2593
pubmed: 28398467
Nat Methods. 2017 Sep;14(9):865-868
pubmed: 28759029
Science. 1998 Apr 24;280(5363):582-5
pubmed: 9554848
Nat Rev Genet. 2019 Jun;20(6):317
pubmed: 30980030
Sci Immunol. 2021 Feb 12;6(56):
pubmed: 33579751
Nat Methods. 2021 Jan;18(1):15-18
pubmed: 33408402