Sublingual Microcirculation Specificity of Sickle Cell Patients: Morphology of the Microvascular Bed, Blood Rheology, and Local Hemodynamics.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
11 Feb 2023
Historique:
received: 12 01 2023
revised: 02 02 2023
accepted: 06 02 2023
entrez: 25 2 2023
pubmed: 26 2 2023
medline: 3 3 2023
Statut: epublish

Résumé

Patients with sickle cell disease (SCD) have poorly deformable red blood cells (RBC) that may impede blood flow into microcirculation. Very few studies have been able to directly visualize microcirculation in humans with SCD. Sublingual video microscopy was performed in eight healthy (HbAA genotype) and four sickle cell individuals (HbSS genotype). Their hematocrit, blood viscosity, red blood cell deformability, and aggregation were individually determined through blood sample collections. Their microcirculation morphology (vessel density and diameter) and microcirculation hemodynamics (local velocity, local viscosity, and local red blood cell deformability) were investigated. The De Backer score was higher (15.9 mm

Identifiants

pubmed: 36835032
pii: ijms24043621
doi: 10.3390/ijms24043621
pmc: PMC9967909
pii:
doi:

Substances chimiques

Hemoglobin, Sickle 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Sachi Sant (S)

Department of Mechanical Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.

Etienne Gouraud (E)

Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM) EA7424, Team «Vascular Biology and Red Blood Cell», Université Claude Bernard Lyon 1, 69008 Lyon, France.
Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, 75015 Paris, France.

Camille Boisson (C)

Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM) EA7424, Team «Vascular Biology and Red Blood Cell», Université Claude Bernard Lyon 1, 69008 Lyon, France.
Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, 75015 Paris, France.
Service de Biochimie et Biologie Moléculaire, Laboratoire de Biologie Médicale Multi-Site, Hospices Civils de Lyon, 69008 Lyon, France.

Elie Nader (E)

Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM) EA7424, Team «Vascular Biology and Red Blood Cell», Université Claude Bernard Lyon 1, 69008 Lyon, France.
Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, 75015 Paris, France.

Mounika Goparaju (M)

Department of Mechanical Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.

Giovanna Cannas (G)

Service de Médecine Interne, Hôpital Edouard Herriot, Hospices Civils de Lyon, 69008 Lyon, France.

Alexandra Gauthier (A)

Institut Hématologique Oncologique Pédiatrique (IHOPe), Hospices Civils de Lyon, 69008 Lyon, France.

Philippe Joly (P)

Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM) EA7424, Team «Vascular Biology and Red Blood Cell», Université Claude Bernard Lyon 1, 69008 Lyon, France.
Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, 75015 Paris, France.
Service de Biochimie et Biologie Moléculaire, Laboratoire de Biologie Médicale Multi-Site, Hospices Civils de Lyon, 69008 Lyon, France.

Céline Renoux (C)

Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM) EA7424, Team «Vascular Biology and Red Blood Cell», Université Claude Bernard Lyon 1, 69008 Lyon, France.
Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, 75015 Paris, France.
Service de Biochimie et Biologie Moléculaire, Laboratoire de Biologie Médicale Multi-Site, Hospices Civils de Lyon, 69008 Lyon, France.

Salima Merazga (S)

Service de Médecine Interne, Hôpital Edouard Herriot, Hospices Civils de Lyon, 69008 Lyon, France.

Christophe Hautier (C)

Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM) EA7424, Team «Vascular Biology and Red Blood Cell», Université Claude Bernard Lyon 1, 69008 Lyon, France.

Philippe Connes (P)

Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM) EA7424, Team «Vascular Biology and Red Blood Cell», Université Claude Bernard Lyon 1, 69008 Lyon, France.
Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, 75015 Paris, France.

Marianne Fenech (M)

Department of Mechanical Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.

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Classifications MeSH