Improved 2α-Hydroxylation Efficiency of Steroids by CYP154C2 Using Structure-Guided Rational Design.


Journal

Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801

Informations de publication

Date de publication:
29 03 2023
Historique:
medline: 31 3 2023
pubmed: 28 2 2023
entrez: 27 2 2023
Statut: ppublish

Résumé

Cytochrome P450 enzymes are promising biocatalysts for industrial use because they catalyze site-selective C-H oxidation and have diverse catalytic reactions and a broad substrate range. In this study, the 2α-hydroxylation activity of CYP154C2 from Streptomyces avermitilis MA-4680T toward androstenedione (ASD) was identified by an

Identifiants

pubmed: 36847541
doi: 10.1128/aem.02186-22
pmc: PMC10056965
doi:

Substances chimiques

Steroids 0
Cytochrome P-450 Enzyme System 9035-51-2
Testosterone 3XMK78S47O

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0218622

Références

Biosci Biotechnol Biochem. 2006 Jan;70(1):307-11
pubmed: 16428858
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
pubmed: 16369096
J Am Chem Soc. 2019 Jun 5;141(22):8989-8995
pubmed: 31070908
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
J Am Chem Soc. 2007 Oct 31;129(43):12940-1
pubmed: 17915876
Chembiochem. 2018 May 18;19(10):1066-1077
pubmed: 29512903
Nat Chem. 2011 Aug 14;3(9):738-43
pubmed: 21860465
J Biol Chem. 2003 Apr 4;278(14):12214-21
pubmed: 12519772
Acta Crystallogr D Struct Biol. 2018 Mar 1;74(Pt 3):215-227
pubmed: 29533229
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
Proc Natl Acad Sci U S A. 2015 Mar 3;112(9):2847-52
pubmed: 25691737
Structure. 2004 Nov;12(11):1921-2
pubmed: 15530355
J Biol Chem. 2010 Dec 3;285(49):38270-82
pubmed: 20889498
J Am Chem Soc. 2014 Mar 5;136(9):3640-6
pubmed: 24521145
J Steroid Biochem Mol Biol. 2015 Jun;150:1-10
pubmed: 25746800
Biochem Biophys Res Commun. 2020 Feb 5;522(2):355-361
pubmed: 31767148
J Biol Chem. 2009 Apr 10;284(15):9937-46
pubmed: 19234303
FEBS J. 2019 May;286(9):1683-1699
pubmed: 30552795
Angew Chem Int Ed Engl. 2020 Jul 20;59(30):12499-12505
pubmed: 32243054
Biochim Biophys Acta. 2007 Mar;1770(3):390-401
pubmed: 16920266
Steroids. 2018 Aug;136:76-92
pubmed: 29360535
Toxicol Lett. 2018 Aug;292:39-45
pubmed: 29702199
Best Pract Res Clin Obstet Gynaecol. 2016 Nov;37:98-118
pubmed: 27387253
Biochemistry. 2008 Nov 18;47(46):11964-72
pubmed: 18937506
Microb Cell Fact. 2013 Oct 17;12:95
pubmed: 24134652
J Biol Chem. 1964 Jul;239:2370-8
pubmed: 14209971
Trends Microbiol. 2020 Jun;28(6):445-454
pubmed: 32396826
J Biol Chem. 2010 May 28;285(22):16844-53
pubmed: 20375018
Appl Microbiol Biotechnol. 2004 Apr;64(3):317-25
pubmed: 14716467
Chembiochem. 2016 Jan 1;17(1):90-101
pubmed: 26478560
Nucleic Acids Res. 2022 May 24;:
pubmed: 35610055
J Comput Chem. 2010 Jan 30;31(2):455-61
pubmed: 19499576
J Am Chem Soc. 2022 Mar 23;144(11):4739-4745
pubmed: 35258294
Nat Prod Rep. 2017 Aug 30;34(9):1141-1172
pubmed: 28758170
Am J Obstet Gynecol. 1981 Aug 1;140(7):815-30
pubmed: 7258262
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Biol Pharm Bull. 2012;35(6):844-9
pubmed: 22687473
Arch Pharm Res. 2015 Jan;38(1):98-107
pubmed: 24988988
Appl Environ Microbiol. 2014 Feb;80(4):1371-9
pubmed: 24334658
Nat Prod Rep. 2012 Oct;29(10):1251-66
pubmed: 22820933
Acta Crystallogr D Biol Crystallogr. 2014 Nov;70(Pt 11):2875-89
pubmed: 25372679
Appl Environ Microbiol. 2019 Nov 14;85(23):
pubmed: 31540985
FEBS J. 2016 Nov;283(22):4128-4148
pubmed: 27686671
J Am Chem Soc. 2020 Jun 10;142(23):10279-10283
pubmed: 32450692

Auteurs

Qilin Gao (Q)

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

Bingbing Ma (B)

Research Center for Clinical Pharmacy, The First Affiliated Hospital & Institute of Pharmaceutical Biotechnology, School of Medicine, Zhejiang University, Hangzhou, China.

Qianwen Wang (Q)

Ocean College, Zhejiang University, Zhoushan, China.

Hao Zhang (H)

Drug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

Shinya Fushinobu (S)

Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan.
Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan.

Jian Yang (J)

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

Susu Lin (S)

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

Keke Sun (K)

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

Bing-Nan Han (BN)

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

Lian-Hua Xu (LH)

College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.

Articles similaires

A molecular mechanism for bright color variation in parrots.

Roberto Arbore, Soraia Barbosa, Jindich Brejcha et al.
1.00
Animals Feathers Pigmentation Parrots Aldehyde Dehydrogenase
Humans Male Testosterone Adult Middle Aged
Saccharomyces cerevisiae Aldehydes Biotransformation Flavoring Agents Lipoxygenase

Mutational analysis of Phanerochaete chrysosporium´s purine transporter.

Mariana Barraco-Vega, Manuel Sanguinetti, Gabriela da Rosa et al.
1.00
Phanerochaete Fungal Proteins Purines Aspergillus nidulans DNA Mutational Analysis

Classifications MeSH